Low-potential immunosensor-based detection of the vascular growth factor 165 (VEGF 165 ) using the nanocomposite platform of cobalt metal-organic framework.

Autor: Singh S; School of Pharmacy, Sharda University Greater Noida 201310 Uttar Pradesh India., Numan A; State Key Laboratory of ASIC and System, SIST, Fudan University 200433 Shanghai China., Zhan Y; State Key Laboratory of ASIC and System, SIST, Fudan University 200433 Shanghai China., Singh V; School of Pharmacy, Sharda University Greater Noida 201310 Uttar Pradesh India., Alam A; Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University Al-Kharj Kingdom of Saudi Arabia., Van Hung T; Institute of Research and Development, Duy Tan University Danang 550000 Vietnam tranvanhung9@duytan.edu.vn nguyendangnam@duytan.edu.vn.; The Faculty of Environmental and Chemical Engineering, Duy Tan University Danang 550000 Vietnam., Nam ND; Institute of Research and Development, Duy Tan University Danang 550000 Vietnam tranvanhung9@duytan.edu.vn nguyendangnam@duytan.edu.vn.; The Faculty of Environmental and Chemical Engineering, Duy Tan University Danang 550000 Vietnam.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2020 Jul 21; Vol. 10 (46), pp. 27288-27296. Date of Electronic Publication: 2020 Jul 21 (Print Publication: 2020).
DOI: 10.1039/d0ra03181j
Abstrakt: The vascular endothelial growth factor 165 (VEGF 165 ) is a quintessential biomarker in cancers. An easy and precise tool for the early detection of malignancies is required for rapid care and metastasis prevention. Cobalt-based metal-organic framework (Co-BTC-GO-MOF) nanoparticles have been used as a signal carrier for the anti-VEGF 165 signaling antibody. Cobalt-based MOF was synthesized using cobalt (Co), benzene-1,3,5-tricarboxylate (BTC), and graphene oxide (GO) applying a hydrothermal method. Structure, compositions, size and morphology of the qualified sensor are determined by using distinctive analytical techniques. The Co-MOF nanoparticles are found to be thermostable, as revealed by thermal stability assay. The strategy utilises an impedimetric and differential pulse voltammetry (DPV) techniques in the presence of the [Fe(CN) 6 ] 3-/4- redox system. Compared to earlier results, this assay resulted in higher sensitivity with the limit of detection (LOD) found to be 5.23 pM in a 0.01 M buffer solution of pH 7.4 using linear scale voltammetry at room temperature. The resulting Co-BTC-GO-MOF immunosensor shows high responsiveness and selectivity in detecting VEGF 165 in real-time serum samples of cancer patients. The electrochemical performance studies confirm that the intended proposed immunosensor could pave the way for the future advancement of high-performance, sensitive, reproducible and robust immunosensors for the cost-effective and initial phase detection of cancer in the future.
Competing Interests: There is no conflict of interest and disclosures associated with the manuscript.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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