Y-body detection in formalin-fixed lymph nodes, using proteolytic enzymes and oxidative deamination.

Autor: Thomsen JL, Lisse IM, Hollaender NH
Jazyk: angličtina
Zdroj: Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology [Acta Pathol Microbiol Immunol Scand A] 1987 Mar; Vol. 95 (2), pp. 103-6.
DOI: 10.1111/j.1699-0463.1987.tb00014_95a.x
Abstrakt: The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine-T and Sodiumhypochlorite) on formalin-fixed paraffin-embedded lymph nodes with regard to detection of fluorescent Y-bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y-body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.
Databáze: MEDLINE