Low-cost, open-source cell culture chamber for regulating physiologic oxygen levels.

Autor: Marchus CRN; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States., Knudson JA; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States., Morrison AE; University of Idaho, Department of Electrical and Computer Engineering, Moscow, ID, United States., Strawn IK; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States., Hartman AJ; University of Idaho, Department of Electrical and Computer Engineering, Moscow, ID, United States., Shrestha D; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States., Pancheri NM; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States., Glasgow I; University of Idaho, Department of Mechanical Engineering, Moscow, ID, United States., Schiele NR; University of Idaho, Department of Chemical & Biological Engineering, Moscow, ID, United States.
Jazyk: angličtina
Zdroj: HardwareX [HardwareX] 2021 Dec 18; Vol. 11, pp. e00253. Date of Electronic Publication: 2021 Dec 18 (Print Publication: 2022).
DOI: 10.1016/j.ohx.2021.e00253
Abstrakt: The physiological oxygen levels for several mammalian cell types in vivo are considered to be hypoxic (low oxygen tension), but the vast majority of in vitro mammalian cell culture is conducted at atmospheric oxygen levels of around 21%. In order to understand the impact of low oxygen environments on cells, oxygen levels need to be regulated during in vitro culture. Two common methods for simulating a hypoxic environment are through the regulation of gas composition or chemical induction. Chemically mimicking hypoxia can have adverse effects such as reducing cell viability, making oxygen regulation in cell culture chambers crucial for long-term culture. However, oxygen-regulating cell culture incubators and commercial hypoxia chambers may not always be a viable option due to cost and limited customization. Other low-cost chambers have been developed, but they tend to lack control systems or are fairly small scale. Thus, the objective of this project was to design and develop a low-cost, open-source, controllable, and reproducible hypoxia chamber that can fit inside a standard cell culture incubator. This design allows for the control of O 2 between 1 and 21%, while maintaining CO 2 levels at 5%, as well as monitoring of temperature, pressure, and relative humidity. Testing showed our hypoxia chamber was able to maintain CO 2 levels at 5% and hypoxic O 2 levels at 1% and 5% for long-term cell culture. This simple and easy-to-manufacture design uses off the shelf components, and the total material cost was $832.47 (USD).
Competing Interests: The authors whose names are listed certify that they have NO affiliations with or involvement in any organization or entity with any financial interest.
(© 2021 The Author(s).)
Databáze: MEDLINE
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