Analyzing the Distribution of Specialized Metabolites from Plant Native Tissues with Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging.

Autor:	Moreno-Pedraza A; Department of Biotechnology and Biochemistry, Center for Research and Advanced Studies (CINVESTAV), Irapuato, Gto, Mexico., Garcia-Rojas NS; Department of Biotechnology and Biochemistry, Center for Research and Advanced Studies (CINVESTAV), Irapuato, Gto, Mexico., Winkler R; Department of Biotechnology and Biochemistry, Center for Research and Advanced Studies (CINVESTAV), Irapuato, Gto, Mexico. robert.winkler@cinvestav.mx.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2469, pp. 145-154.
DOI:	10.1007/978-1-0716-2185-1_12
Abstrakt:	The localization of metabolites in plant tissues is often related to their biological function and biosynthesis. Mass spectrometry imaging (MSI) provides comprehensive information about the distribution of known and unknown compounds in tissues. In this protocol, we describe the use of laser desorption low-temperature plasma (LD-LTP) ionization MSI. This technology enables the direct analysis of native tissues under ambient conditions. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu