Extraction and Analysis of Phenolic Compounds from Grape Berries.

Autor:	Tyagi K; Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel.; Department of Viticulture and Enology, University of California, Davis, CA, USA., Lerno L; Department of Viticulture and Enology, University of California, Davis, CA, USA.; Food Safety and Measurement Facility, University of California, Davis, CA, USA., De Rosso M; Chemistry Lab., Council for Agricultural Research and Economics - Viticulture & Enology (CREA-VE), Conegliano, Italy., Maoz I; Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel., Lichter A; Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel., Ebeler SE; Department of Viticulture and Enology, University of California, Davis, CA, USA. seebeler@ucdavis.edu., Flamini R; Chemistry Lab., Council for Agricultural Research and Economics - Viticulture & Enology (CREA-VE), Conegliano, Italy. riccardo.flamini@crea.gov.it.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2469, pp. 1-17.
DOI:	10.1007/978-1-0716-2185-1_1
Abstrakt:	Phenolics are ubiquitous compounds that represent the most abundant and diverse class of plant metabolites. From an analytical point of view, phenolics can be divided into soluble phenolics such as phenolic acids, phenylpropanoids, flavonoids and quinones, and nonsoluble compounds such as proanthocyanidins, lignins, and cell wall-bound hydroxycinnamic acids. Extraction of phenolics from the sample material is the first step toward their analysis. Biochemical methods for determination of total phenolics content were widely used in the past but modern chromatographic and mass spectrometric methods for identification and quantification of individual compounds are available in recent years. In this chapter, we describe methods for phenolic compounds extraction used in our laboratories from berries of Vitis vinifera and analytical methods including HPLC coupled to DAD detector and Q-TOF LC/MS for their analysis. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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