Efficient gene transfection to lung cancer cells via Folate-PEI-Sorbitol gene transporter.

Autor: Cho KS; Department of Tumor Immunology, National Cancer Center, Goyang, Republic of Korea.; Department of Internal Medicine, Yonsei University College of Medical Science, Seoul, Republic of Korea., Kim S; Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Chun HB; Department of Life Science, Waterloo University, Waterloo, Ontario, Canada., Cheon JH; Department of Internal Medicine, Yonsei University College of Medical Science, Seoul, Republic of Korea., Cho MH; RNABIO, Seongnam, Gyeonggi-do, Republic of Korea., Lee AY; Department of Life Science, Waterloo University, Waterloo, Ontario, Canada., Arote RB; Department of Molecular Genetics & Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 May 04; Vol. 17 (5), pp. e0266181. Date of Electronic Publication: 2022 May 04 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0266181
Abstrakt: Lung cancer is known to be one of the fatal diseases in the world and is experiencing treatment difficulties. Many treatments have been discovered and implemented, but death rate of patients with lung cancer continues to remain high. Current treatments for cancer such as chemotherapy, immunotherapy, and radiotherapy have shown considerable results, yet they are accompanied by side effects. One effective method for reducing the cytotoxicity of these treatments is via the use of a nanoparticle-mediated siRNA delivery strategy with selective silencing effects and non-viral vectors. In this study, a folate (FA) moiety ligand-conjugated poly(sorbitol-co-PEI)-based gene transporter was designed by combining low-molecular weight polyethyleneimine (LMW PEI) and D-sorbitol with FA to form FPS. Since folate receptors are commonly overexpressed in various cancer cells, folate-conjugated nanoparticles may be more effectively delivered to selective cancer cells. Additionally, siOPA1 was used to induce apoptosis through mitochondrial fusion. The OPA1 protein stability level is important for maintaining normal mitochondrial cristae structure and function, conserving the inner membrane structure, and protecting cells from apoptosis. Consequently, when FPS/siOPA1 was used for lung cancer in-vitro and in-vivo, it improved cell viability and cellular uptake.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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