A panoptic review of techniques for finfish disease diagnosis: The status quo and future perspectives.

Autor: Johny TK; Peninsular and Marine Fish Genetic Resources Centre, ICAR-NBFGR, CMFRI Campus, Kochi, Kerala 682018, India., Swaminathan TR; Peninsular and Marine Fish Genetic Resources Centre, ICAR-NBFGR, CMFRI Campus, Kochi, Kerala 682018, India. Electronic address: rajanbfgr@gmail.com., Sood N; ICAR National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow, Uttar Pradesh 226002, India., Pradhan PK; ICAR National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow, Uttar Pradesh 226002, India., Lal KK; ICAR National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow, Uttar Pradesh 226002, India.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2022 May; Vol. 196, pp. 106477. Date of Electronic Publication: 2022 May 02.
DOI: 10.1016/j.mimet.2022.106477
Abstrakt: Disease outbreaks caused by bacterial and viral pathogens is a major impediment to the sustainable growth of aquaculture. Rapid and accurate diagnosis of pathogens is crucial for the successful maintenance of fish health and productivity in aquaculture. This review manuscript provides a brief description of conventional disease diagnosis techniques and a detailed description of immunological techniques such as ELISA, immunofluorescence, immunohistochemistry and lateral flow immunoassay. Specific emphasis has been given to detail the molecular techniques, such as PCR and its variants, including the novel isothermal amplification techniques like LAMP and RPA, that can cater to the need of rapid and sensitive point-of-care diagnostics. Hybridization-based methods and molecular typing methods have also been discussed as they find specific applications in diagnostics. The potential of novel techniques such as MALDI-TOF-MS, flow cytometry, and nanotechnology-based methods have also been outlined as they are likely to revolutionise disease diagnosis in the future. This manuscript provides an update on the principle, strengths, weaknesses, applications and variations of each technique, so as to eliminate the qualms for the adoption of these techniques in aquaculture diagnostics.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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