Poly-L-lysine-modified with ferrocene to obtain a redox polymer for mediated glucose biosensor application.

Autor: Estrada-Osorio DV; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, 76010 Santiago de Querétaro, Mexico., Escalona-Villalpando RA; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, 76010 Santiago de Querétaro, Mexico. Electronic address: ricardo.escalona@uaq.edu.mx., Gutiérrez A; Tecnológico de Estudios Superiores de Huixquilucan, Paraje El Río S/N, La Magdalena Chichicaspa, 52773 Huixquilucan, Edo. de México, Mexico., Arriaga LG; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, 76703 Querétaro, Mexico., Ledesma-García J; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, 76010 Santiago de Querétaro, Mexico. Electronic address: janet.ledesma@uaq.mx.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2022 Aug; Vol. 146, pp. 108147. Date of Electronic Publication: 2022 Apr 27.
DOI: 10.1016/j.bioelechem.2022.108147
Abstrakt: Ferrocene-based polymers as redox mediators are considered versatile and important in the study of glucose biosensors. Poly-L-lysine (PLL), as a cationic polymer, possesses good properties including biocompatibility, biodegradation and water solubility. In this work, PLL was modified with ferrocene carboxylate in a very simple way by activating the carboxyl group of Fc, which reacted with the amino groups of the polymer. The resulting product was analysed by FTIR. Performance as a redox mediator (Fc-PLL) with the enzyme glucose oxidase was tested by cyclic voltammetry and showed an increase in the oxidation current in the presence of glucose in PBS pH 7.4. Additionally, performance as a biosensor was evaluated by amperometry and gave a linear range of 0-10 mM, a limit of detection of 23 μM, a sensitivity of 6.55 μA/cm 2 mM and high selectivity. To evaluate the charged regions of Fc-PLL/GOx on the electrode surface, analysis by scanning electrochemical microscopy showed remarkable activity. The Fc-PLL redox polymer as a glucose biosensor has been well accepted as this kind of material, and the results showed remarkable activity as an electron transfer mediator between the redox polymer and the GOx enzyme.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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