The Role of Long Non-Coding RNA CCAT1 and SNHG14 in Activation of Some Protein-Coding Genes Associated with the Development of Ovarian Cancer.

Autor: Brovkina OI; Research Institute of General Pathology and Pathophysiology, Moscow, Russia. brov.olia@gmail.com.; Federal Research Clinical Center of Specialized Types of Medical Care and Medical Technologies, Federal Medical-Biological Agency of Russia, Moscow, Russia. brov.olia@gmail.com., Pronina IV; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Burdennyy AM; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Uroshlev LA; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.; N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia., Filippova EA; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Fridman MV; N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia., Kazubskaya TP; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Zhordania KI; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Loginov VI; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Kushlinskii NE; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Braga EA; Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Apr; Vol. 172 (6), pp. 760-764. Date of Electronic Publication: 2022 May 02.
DOI: 10.1007/s10517-022-05473-8
Abstrakt: Late diagnosis of ovarian cancer is one of the most important problems in its treatment. Long non-coding RNA (lncRNA) are a poorly studied, but promising type of diagnostic biomarkers. We studied the lncRNA interactome to identify biomarkers with potential significance for molecular diagnostics of ovarian cancer. By screening the TCGA database, we identified differentially expressed lncRNA CCAT1 and SNHG14. Based on the indices of complementarity of CCAT1 and SNHG14 to the mRNA sequences, we selected 5 protein-coding genes MAPK1, c-MET, TGFB2, SNAIL1, and WNT4 associated with the epithelial-mesenchymal transition. Real-time PCR on 54 ovarian cancer samples confirmed the high expression levels of CCAT1 and SNHG14 (logFC>1.5, p<0.05). A positive correlation between the expression levels of two lncRNA and mRNA of 5 genes in 6 pairs was established. The activating effect of CCAT1 and SNHG14 on the expression of these genes can be mediated by miR-203 and miR-124.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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