| Autor: |
Mikhailova MM; National Research Centre «Kurchatov Institute», Moscow, Russia., Sydoruk KV; National Research Centre «Kurchatov Institute», Moscow, Russia.; National Research Centre «Kurchatov Institute» - GosNIIGenetika, Moscow, Russia., Davydova LI; National Research Centre «Kurchatov Institute», Moscow, Russia.; National Research Centre «Kurchatov Institute» - GosNIIGenetika, Moscow, Russia., Yastremsky EV; National Research Centre «Kurchatov Institute», Moscow, Russia.; Shubnikov Institute of Crystallography of FSRC 'Crystallography and Photonics' RAS, Moscow, Russia., Chvalun SN; National Research Centre «Kurchatov Institute», Moscow, Russia., Debabov VG; National Research Centre «Kurchatov Institute», Moscow, Russia.; National Research Centre «Kurchatov Institute» - GosNIIGenetika, Moscow, Russia., Bogush VG; National Research Centre «Kurchatov Institute», Moscow, Russia.; National Research Centre «Kurchatov Institute» - GosNIIGenetika, Moscow, Russia., Panteleyev AA; National Research Centre «Kurchatov Institute», Moscow, Russia. |
| Abstrakt: |
Recombinant spidroins (RS; the analogues of silk proteins of spider's web) have multiple properties beneficial for bioengineering, including their suitability for electrospinning and thus, for production of materials with oriented fibers. This makes RS-based matrices potentially effective in stimulating regeneration of peripheral nerves. The restoration of injured nerves also depends on prompt regrowth of blood vessels. Therefore, prospective scaffold materials for neuro-regenerative therapy should positively affect both the nerves and the blood vessels. Currently, the experimental models suitable for culturing and quantitative assessment of the vascular and neuronal cells on the same material are lacking. Here, we assessed the suitability of electrospun RS-based matrices for cultivation of the mouse aorta and dorsal root ganglia (DRG) explants. We also quantified the effects of matrix topography upon both types of tissues. The RS-based materials have effectively supported aortic explants survival and sprouting. The cumulative length of endothelial sprouts on rS1/9-coated inserts was significantly higher as compared to type I collagen coatings, suggesting stimulatory effects on angiogenesis in vitro. In contrast to matrices with random fibers, on matrices with parallel fibers the migration of both smooth muscle and endothelial cells was highly oriented. Furthermore, alignment of RS fibers effectively directs the growth of axons and the migration of Schwann cells from DRGs. Thus, the electrospun RS matrices are highly suitable to culture both, the DRGs and aortic explants and to study the effects of matrix topography on cell migration. This model has a high potential for further endeavor into interactions of nerve and vascular cells and tissues. |
