In vitro assessment of the bioactivities of sericin protein extracted from a bacterial silk-like biopolymer.

Autor: El-Fakharany EM; Proteins Research Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt esmailelfakharany@yahoo.co.uk., Abu-Elreesh GM; Environmental Biotechnology Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt abdelhaleemm@yahoo.de., Kamoun EA; Polymeric Materials Research Dep., Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications New Borg Al-Arab City 21934 Alexandria Egypt.; Nanotechnology Research Center (NTRC), The British University in Egypt (BUE) El-Sherouk City Cairo 11837 Egypt., Zaki S; Environmental Biotechnology Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt abdelhaleemm@yahoo.de., Abd-El-Haleem DA; Environmental Biotechnology Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt abdelhaleemm@yahoo.de.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2020 Jan 31; Vol. 10 (9), pp. 5098-5107. Date of Electronic Publication: 2020 Jan 31 (Print Publication: 2020).
DOI: 10.1039/c9ra09419a
Abstrakt: Sericin is one of the main components of silk proteins, which has numerous biomedical applications because of its antioxidant, anticancer and antimicrobial properties. We recently isolated and characterized a novel silk-like protein named BNES. It is of non-animal origin and is like a bacterial polymeric silk. Sericin is a very popular protein compound that is effective in treating cancerous tumors. The process of extracting it from natural silk produced by silkworms or spiders is both complex and expensive. From the published scientific literature, it has been shown that sericin has not been previously extracted from a bacterial source. In the present study, sericin was extracted from bacteria capable of producing a biopolymer named BNES whose chemical composition is like that of natural silk and its bio-therapeutic effects were evaluated for the first time. The antioxidant activity of BNES measured by DPPH and ABTS assays showed IC 50 values of 0.38 and 0.41 mg mL -1 , respectively. BNES displayed satisfactory cytotoxic effect against four cancer cell lines, including Huh-7 , Caco-2 , MCF-7 and A549 cells, with IC 50 values in the ranges of ca. 0.62 ± 0.17, 0.72 ± 0.27, 0.76 ± 0.36 and 0.83 ± 0.31 mg mL -1 , respectively, after 24 h of treatment and 0.51 ± 0.22, 0.49 ± 0.19, 0.41 ± 0.25 and 0.55 ± 0.38, respectively, after 48 h of treatment, without affecting normal cells ( WI38 cells). The antitumor activity of BNES was established to be an apoptosis-dependent mechanism determined via cellular morphology alterations, cell cycle arrest in the sub-G1 phase and nuclear staining with highly fluorescent fragments. The antimicrobial effects of BNES were examined with yeast and Gram-negative and Gram-positive bacteria. The results confirmed its antimicrobial activity against all tested organisms at concentrations of up to 1.33 mg mL -1 . The competitive advantage of the bacterial sericin BNES over sericin extracted from spider or silkworm sources is that it can be produced in very large quantities through large-scale bio-fermenters, which reduces the expected cost of production, in addition to having sustainable bacterial production source.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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