Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans.

Autor: Zihad SMNK; Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.; Department of Pharmacy, State University of Bangladesh, Dhaka 1205, Bangladesh.; Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Hasan MT; Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh., Sultana MS; Agrotechnology Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh., Nath S; Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK., Nahar L; Laboratory of Growth Regulators, Institute of Experimental Botany ASCR, Palacký University, Šlechtitelů 27, Olomouc 78371, Czech Republic., Rashid MA; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh., Uddin SJ; Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh., Sarker SD; Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK., Shilpi JA; Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2022 Apr 22; Vol. 2022, pp. 9600079. Date of Electronic Publication: 2022 Apr 22 (Print Publication: 2022).
DOI: 10.1155/2022/9600079
Abstrakt: The Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia officinalis collected from the Sundarbans. Five of them were identified as Aspergillus sp. and one as Penicillium sp. by macroscopic and microscopic observation. Antibacterial activity of the crude extracts obtained from these endophytes was determined against Staphylococcus aureus , Micrococcus luteus , Escherichia coli , and Pseudomonas aeruginosa using resazurin-based microtiter assay. The isolated endophytes showed varying degrees of antibacterial activity with MICs ranging between 5 and 0.078 mg/mL. Molecular identification of the most active endophyte revealed its identity as Aspergillus fumigatus obtained from the leaves of C . decandra . Acute toxicity study of the ethyl acetate extract of A . fumigatus in mice revealed no mortality even at the highest dose of 2000 mg/kg bodyweight, though some opposing results are found in the subacute toxicity study. The extract was subjected to silica gel and Sephadex column chromatography resulting in the isolation of three pure compounds. LC-MS analysis of these pure compounds revealed their identity as fumigaclavine C, azaspirofuran B, and fraxetin. This is the first report of fraxetin from A . fumigatus . All three identified compounds were previously reported for their antibacterial activity against different strains of both Gram-positive and Gram-negative bacteria. Therefore, the observed antibacterial activity of the ethyl acetate (EtOAc) extract of A . fumigatus could be due to the presence of these compounds. These results support the notion of investigating fungal endophytes from the Sundarbans for new antimicrobial compounds.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2022 S. M. Neamul Kabir Zihad et al.)
Databáze: MEDLINE
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