| Autor: |
Brossat B, Straczek J, Heulin MH, Herbeuval X, Belleville F, Nabet P, Lokiec F |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1987 Jan 23; Vol. 413, pp. 141-50. |
| DOI: |
10.1016/0378-4347(87)80221-9 |
| Abstrakt: |
A simple reliable liquid chromatographic method for assay of cyclosporin A in serum or urine is described. Samples were cleaned up on a solid-phase extraction system (cyanopropyl column). The system involved a reversed-phase C18 Ultrasphere column maintained at 72 degrees C and an acetonitrile linear gradient (65 to 95%) in 0.14% triethylammonium phosphate. Liquid chromatographic analysis of radioimmunoassay standards shows that some samples contain a contaminant peak. Comparison of cyclosporin A levels obtained by radioimmunoassay and high-performance liquid chromatography in clinical investigations show that the former values are generally, but not always, higher than the latter, and that cyclosporin A is very differently metabolized depending on the patient, disease and treatment. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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