Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis.

Autor:	Shpichka AI; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638., Konarev PV; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru.; National Research Center 'Kurchatov Institute' Moscow Russia., Efremov YM; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638., Kryukova AE; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru.; National Research Center 'Kurchatov Institute' Moscow Russia., Aksenova NA; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638.; Department of Polymers and Composites, N. N. Semenov Institute of Chemical Physics Moscow Russia., Kotova SL; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638.; Department of Polymers and Composites, N. N. Semenov Institute of Chemical Physics Moscow Russia., Frolova AA; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638., Kosheleva NV; FSBSI 'Institute of General Pathology and Pathophysiology' Moscow Russia.; Faculty of Biology, Lomonosov Moscow State University Moscow Russia., Zhigalina OM; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru.; Bauman Moscow State Technical University Moscow Russia., Yusupov VI; Institute of Photon Technologies, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia iouss@yandex.ru., Khmelenin DN; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru., Koroleva A; Laser Zentrum Hannover e. V. Hannover Germany info.koroleva@gmail.com., Volkov VV; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru., Asadchikov VE; A. V. Shubnikov Institute of Crystallography, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia peter_konarev@mail.ru krukovaae@yandex.ru zhigal@crys.ras.ru dirq@rambler.ru volkicras@mail.ru asad@crys.ras.ru., Timashev PS; Institute for Regenerative Medicine, Sechenov University 2-8 Trubetskaya St. Moscow Russia 119991 ana-shpichka@yandex.ru yu.efremov@gmail.com naksenova@mail.ru slkotova@mail.ru nastyfr@ya.ru timashev.peter@gmail.com +7 495 6091400 ext. 3638.; Department of Polymers and Composites, N. N. Semenov Institute of Chemical Physics Moscow Russia.; Institute of Photon Technologies, Federal Scientific Research Center 'Crystallography and Photonics' RAS Moscow Russia iouss@yandex.ru.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2020 Jan 24; Vol. 10 (8), pp. 4190-4200. Date of Electronic Publication: 2020 Jan 24 (Print Publication: 2020).
DOI:	10.1039/c9ra08169k
Abstrakt:	Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve its properties remains undiscussed despite its importance, e.g. in designing biomaterials that ensure cell migration and lumenogenesis. We sought to uncover the structural aspects of PEGylated fibrin hydrogels shown to contribute to angiogenesis. The analysis of the small-angle X-ray scattering (SAXS) data and ab initio modeling revealed that the PEGylation of fibrinogen led to the formation of oligomeric species, which are larger at a higher PEG : fibrinogen molar ratio. The improvement of optical properties was provided by the decrease in aggregates' sizes and also by retaining the bound water. Compared to the native fibrin, the structure of the 5 : 1 PEGylated fibrin gel consisted of homogenously distributed flexible fibrils with a smaller space between them. Moreover, as arginylglycylaspartic acid (RGD) sites may be partly bound to PEG-NHS or masked because of the oligomerization, the number of adhesion sites may be slightly reduced that may provide the better cell migration and formation of continuous capillary-like structures. Competing Interests: The authors declare no competing interests. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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