Genotypic confirmation of a biased phenotypic sex ratio in a dryland moss using restriction fragment length polymorphisms.

Autor: Ekwealor JTB; Department of Integrative Biology and University and Jepson Herbaria University of California Berkeley USA.; Data Science Lab, Office of the Chief Information Officer Smithsonian Institution Washington D.C. USA., Benjamin SD; Department of Biological Sciences California State University Los Angeles, California USA., Jomsky JZ; Department of Integrative Biology and University and Jepson Herbaria University of California Berkeley USA., Bowker MA; School of Forestry Northern Arizona University Flagstaff Arizona USA., Stark LR; School of Life Sciences University of Nevada Las Vegas Nevada USA., McLetchie DN; Department of Biology University of Kentucky Lexington Kentucky USA., Mishler BD; Department of Integrative Biology and University and Jepson Herbaria University of California Berkeley USA., Fisher KM; Department of Biological Sciences California State University Los Angeles, California USA.
Jazyk: angličtina
Zdroj: Applications in plant sciences [Appl Plant Sci] 2022 Apr 17; Vol. 10 (2), pp. e11467. Date of Electronic Publication: 2022 Apr 17 (Print Publication: 2022).
DOI: 10.1002/aps3.11467
Abstrakt: Premise: In dioicous mosses, sex is determined by a single U (female, ♀) or V (male, ♂) chromosome. Although a 1 : 1 sex ratio is expected following meiosis, phenotypic sex ratios based on the production of gametangia are often female-biased. The dryland moss Syntrichia caninervis (Pottiaceae) is notable for its low frequency of sex expression and strong phenotypic female bias. Here we present a technique to determine genotypic sex in a single shoot of S. caninervis , and report results of a case study examining genotypic and phenotypic sex ratios.
Methods: We reanalyzed 271 non-expressing gametophyte shoots from a previous study on S. caninervis sex expression across microhabitats using a restriction fragment length polymorphism (RFLP) method.
Results: We recovered a genotypic sex ratio in non-expressing shoots of 18.4♀ : 1♂, which exceeds the female bias of the phenotypic ratio (5.3♀ : 1♂; P  = 0.013). We also found that the distribution of male and female genotypes across microsites with different levels of sun exposure was not predicted by patterns of sex expression in these microsites.
Discussion: These findings contribute to our understanding of how the environment may modulate sex ratios in S. caninervis , either through its direct influence on sex expression or through selection on genotypes with particular sex expression phenotypes.
(© 2022 The Authors. Applications in Plant Sciences published by Wiley Periodicals LLC on behalf of Botanical Society of America.)
Databáze: MEDLINE
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