A native promoter-gene fusion created by CRISPR/Cas9-mediated genomic deletion offers a transgene-free method to drive oil accumulation in leaves.

Autor: Bhunia RK; Plant Sciences and the Bioeconomy, Rothamsted Research, Harpenden, UK.; National Agri-Food Biotechnology Institute (NABI), Mohali, India., Menard GN; Plant Sciences and the Bioeconomy, Rothamsted Research, Harpenden, UK., Eastmond PJ; Plant Sciences and the Bioeconomy, Rothamsted Research, Harpenden, UK.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2022 Aug; Vol. 596 (15), pp. 1865-1870. Date of Electronic Publication: 2022 May 06.
DOI: 10.1002/1873-3468.14365
Abstrakt: Achieving gain-of-function phenotypes without inserting foreign DNA is an important challenge for plant biotechnologists. Here, we show that a gene can be brought under the control of a promoter from an upstream gene by deleting the intervening genomic sequence using dual-guide CRISPR/Cas9. We fuse the promoter of a nonessential photosynthesis-related gene to DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) in the lipase-deficient sugar-dependent 1 mutant of Arabidopsis thaliana to drive ectopic oil accumulation in leaves. DGAT2 expression is enhanced more than 20-fold and the triacylglycerol content increases by around 30-fold. This deletion strategy offers a transgene-free route to engineering traits that rely on transcriptional gain-of-function, such as producing high lipid forage to increase the productivity and sustainability of ruminant farming.
(© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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