Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea: A cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in Mozambique.

Autor:	Manhique-Coutinho L; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Universidade Eduardo Mondlane, Maputo 3453, Mozambique. Electronic address: lena.coutinho@ins.gov.mz., Chiani P; Istituto Superiore di Sanità, Department of Food Safety, Nutrition and Veterinary Public Health, 299, 0161, Rome, Italy., Michelacci V; Istituto Superiore di Sanità, Department of Food Safety, Nutrition and Veterinary Public Health, 299, 0161, Rome, Italy., Taviani E; Universidade Eduardo Mondlane, Maputo 3453, Mozambique., Bauhofer AFL; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal., Chissaque A; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal., Cossa-Moiane I; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Institute of Tropical Medicine (ITM), 2000 Antwerp, Belgium., Sambo J; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal., Chilaúle J; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique., Guimarães EL; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal., Salência J; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique., Cassocera M; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal., Bero DM; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique., Langa JP; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique., de Deus N; Instituto Nacional de Saúde (INS), Estrada Nacional EN1, Parcela n ͦ 3943, Distrito de Marracuene, Maputo, Mozambique.; Universidade Eduardo Mondlane, Maputo 3453, Mozambique.
Jazyk:	angličtina
Zdroj:	International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2022 Aug; Vol. 121, pp. 190-194. Date of Electronic Publication: 2022 Apr 28.
DOI:	10.1016/j.ijid.2022.04.054
Abstrakt:	Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children aged <15 years with diarrhea in four Mozambican provinces. Methods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambézia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby-Bauer method. Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin-producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%). Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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