Real-time monitoring of epithelial barrier function by impedance spectroscopy in a microfluidic platform.

Autor:	Fernandes J; Electronics and Computer Science, Faculty of Physical Sciences and Engineering, University of Southampton, UK. hm@ecs.soton.ac.uk., Karra N; Electronics and Computer Science, Faculty of Physical Sciences and Engineering, University of Southampton, UK. hm@ecs.soton.ac.uk., Bowring J; Electronics and Computer Science, Faculty of Physical Sciences and Engineering, University of Southampton, UK. hm@ecs.soton.ac.uk., Reale R; Electronics and Computer Science, Faculty of Physical Sciences and Engineering, University of Southampton, UK. hm@ecs.soton.ac.uk., James J; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK., Blume C; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK.; Institute for Life Sciences, University of Southampton, UK.; NIHR Southampton Biomedical Research Centre, University Hospital Southampton, UK., Pell TJ; Novel Human Genetics Research Unit, GlaxoSmithKline R&D, Stevenage, Hertfordshire, UK., Rowan WC; Novel Human Genetics Research Unit, GlaxoSmithKline R&D, Stevenage, Hertfordshire, UK., Davies DE; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK.; Institute for Life Sciences, University of Southampton, UK.; NIHR Southampton Biomedical Research Centre, University Hospital Southampton, UK., Swindle EJ; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK.; Institute for Life Sciences, University of Southampton, UK.; NIHR Southampton Biomedical Research Centre, University Hospital Southampton, UK., Morgan H; Electronics and Computer Science, Faculty of Physical Sciences and Engineering, University of Southampton, UK. hm@ecs.soton.ac.uk.; Institute for Life Sciences, University of Southampton, UK.
Jazyk:	angličtina
Zdroj:	Lab on a chip [Lab Chip] 2022 May 17; Vol. 22 (10), pp. 2041-2054. Date of Electronic Publication: 2022 May 17.
DOI:	10.1039/d1lc01046h
Abstrakt:	A multichannel microfluidic platform for real-time monitoring of epithelial barrier integrity by electrical impedance has been developed. Growth and polarization of human epithelial cells from the airway or gastrointestinal tract was continuously monitored over 5 days in 8 parallel, individually perfused microfluidic chips. Electrical impedance data were continuously recorded to monitor cell barrier formation using a low-cost bespoke impedance analyser. Data was analysed using an electric circuit model to extract the equivalent transepithelial electrical resistance and epithelial cell layer capacitance. The cell barrier integrity steadily increased overtime, achieving an average resistance of 418 ± 121 Ω cm 2 (airway cells) or 207 ± 59 Ω cm 2 (gastrointestinal cells) by day 5. The utility of the polarized airway epithelial barrier was demonstrated using a 24 hour challenge with double stranded RNA to mimic viral infection. This caused a rapid decrease in barrier integrity in association with disruption of tight junctions, whereas simultaneous treatment with a corticosteroid reduced this effect. The platform is able to measure barrier integrity in real-time and is scalable, thus has the potential to be used for drug development and testing.
Databáze:	MEDLINE
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