Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology.

Autor:	Linciano S; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Wong EL; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Mazzocato Y; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Chinellato M; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.; Department of Medicine (DIMED), University of Padua, Padua, Italy., Scaravetti T; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Caregnato A; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Cacco V; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Romanyuk Z; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy., Angelini A; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy. alessandro.angelini@unive.it.; European Centre for Living Technology (ECLT), Ca' Bottacin, Venice, Italy. alessandro.angelini@unive.it.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2491, pp. 251-262.
DOI:	10.1007/978-1-0716-2285-8_14
Abstrakt:	The ability of cross-reactive antibodies to bind multiple related or unrelated targets derived from different species provides not only superior therapeutic efficacy but also a better assessment of treatment toxicity, thereby facilitating the transition from preclinical models to human clinical studies. This chapter provides some guidelines for the directed evolution of cross-reactive antibodies using yeast surface display technology. Cross-reactive antibodies are initially isolated from a naïve library by combining highly avid magnetic bead separations followed by multiple cycles of flow cytometry sorting. Once initial cross-reactive clones are identified, sequential rounds of mutagenesis and two-pressure selection strategies are applied to engineer cross-reactive antibodies with improved affinity and yet retained or superior cross-reactivity. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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