Absolute content determination by quantitative NMR (qNMR) spectroscopy: a curious case of aldosterone.
| Autor: | Singh N; Roche Diagnostics Nonnenwald 2, 82377 Penzberg Germany neeraj.singh.ns1@roche.com christian.geletneky@roche.com., Taibon J; Roche Diagnostics Nonnenwald 2, 82377 Penzberg Germany neeraj.singh.ns1@roche.com christian.geletneky@roche.com., Pongratz S; Roche Diagnostics Nonnenwald 2, 82377 Penzberg Germany neeraj.singh.ns1@roche.com christian.geletneky@roche.com., Geletneky C; Roche Diagnostics Nonnenwald 2, 82377 Penzberg Germany neeraj.singh.ns1@roche.com christian.geletneky@roche.com. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2021 Jul 05; Vol. 11 (38), pp. 23627-23630. Date of Electronic Publication: 2021 Jul 05 (Print Publication: 2021). |
| DOI: | 10.1039/d1ra03472c |
| Abstrakt: | Quantitative NMR spectroscopy has been utilized to calculate the absolute content (g g -1 ) of aldosterone, which is necessary for electrolyte balance and blood pressure regulation, in commercially available materials. Explanations have been provided for many signals observed in the 1 HNMR spectrum, false interpretation of which can have significant effects if such a value is utilized for the primary calibrators in ID-LC-MS/MS ('gold standard') reference methods in clinical chemistry. Competing Interests: All the authors are employees of Roche Diagnostics, Nonnenwald 2, 82377 Penzberg, Germany. There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|