Stochastic biosensors based on N- and S-doped graphene for the enantioanalysis of aspartic acid in biological samples.

Autor:	Stefan-van Staden RI; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com.; Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest Bucharest Romania., Gheorghe DC; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com.; Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest Bucharest Romania., Ilie-Mihai RM; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com., Tudoran LB; National Institute for Research and Development of Isotopic and Molecular Technologies Donat Street 67-103 Cluj-Napoca Romania., Pruneanu SM; National Institute for Research and Development of Isotopic and Molecular Technologies Donat Street 67-103 Cluj-Napoca Romania.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2021 Jul 01; Vol. 11 (38), pp. 23301-23309. Date of Electronic Publication: 2021 Jul 01 (Print Publication: 2021).
DOI:	10.1039/d1ra02066h
Abstrakt:	Metabolomics plays a very important role in cancer mechanism and also in the early diagnosis of cancer. The enantioanalysis of aminoacids found in biological samples may favor the development of new screening tests for the early diagnosis of cancer. Two stochastic biosensors, based on the immobilization of α-hemolysin and hemin in exfoliated reduced graphene modified with nitrogen and sulphur, were proposed for the enantioanalysis of aspartic acid in biological samples (whole blood, urine, saliva, and tissue). The proposed biosensors showed low limits of quantification, high sensitivity, and large linear concentration ranges. The recoveries of d- and l-aspartic acid in biological samples were higher than 95.00%, with a relative standard deviation lower than 1.00%. Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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