Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics.

Autor:	Page R; ImmunoEngineering Lab, School of Cancer and Pharmaceutical Sciences, King's College London, Guy's Cancer Centre, Great Maze Pond, London SE1 9RT, UK., Scourfield E; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Ficarelli M; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., McKellar SW; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Lee KL; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Maguire TJA; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Bouton C; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Lista MJ; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Neil SJD; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Malim MH; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Zuckerman M; South London Specialist Virology Centre, King's College Hospital, London, UK., Mischo HE; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK., Martinez-Nunez RT; Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 Mar 22; Vol. 3 (2), pp. 101300. Date of Electronic Publication: 2022 Mar 22 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101300
Abstrakt:	The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022). Competing Interests: The authors declare no competing interests. (© 2022 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu