Bone marrow transplantation induces changes in the gut microbiota that chronically increase the cytokine response pattern of splenocytes.

Autor: Katiraei S; Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands., van Diepen JA; Department of Internal Medicine, Radboud UMC, Nijmegen, The Netherlands., Tavares LP; Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Hoving LR; Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands., Pronk A; Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands., Verschueren I; Department of Internal Medicine, Radboud UMC, Nijmegen, The Netherlands., Rensen PCN; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands., Zwaginga JJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Kostidis S; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands., Giera M; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands., Teixera M; Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., van Dijk KW; Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands. kowvd@lumc.nl.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands. kowvd@lumc.nl.; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands. kowvd@lumc.nl., Netea MG; Department of Internal Medicine, Radboud UMC, Nijmegen, The Netherlands., Berbée JFP; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands., van Harmelen V; Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Apr 27; Vol. 12 (1), pp. 6883. Date of Electronic Publication: 2022 Apr 27.
DOI: 10.1038/s41598-022-10637-7
Abstrakt: Bone marrow transplantation (BMT) involves conditioning regimens which acutely induce side effects, including systemic inflammation, intestinal damage and shifts in the gut microbial composition, some of which may persist chronically. As the gut microbiota affect systemic immune responses, we aimed to investigate whether, post-BMT, the peripheral immune system is modulated as a direct consequence of alterations in the gut microbiota. We show that 24 weeks post-BMT, splenocytes but not peritoneal macrophages display increased cytokine response patterns upon ex-vivo stimulation with various pathogens as compared to untreated controls. The pattern of BMT-induced cytokine responses was transferred to splenocytes, and not to peritoneal macrophages, of healthy controls via co-housing and transferred to germfree mice via transplantation of cecum content. Thus, BMT induces changes in gut microbiota that in their turn increase cytokine responsiveness of splenocytes. Thus, BMT establishes a dominant microbiota that attenuates normalization of the immune-response.
(© 2022. The Author(s).)
Databáze: MEDLINE
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