Phenotypic Changes in Mammary Adenocarcinoma (4T1) cells In Vitro after Treatment with Carcinosinum.

Autor:	Silva TCD; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil., Santos WAD; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil., Pinto SAG; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil., Rocha PRD; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil., Hurtado ECP; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil., Bonamin LV; Research Center, Graduation Program in Environmental and Experimental Pathology, University Paulista-UNIP, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Homeopathy : the journal of the Faculty of Homeopathy [Homeopathy] 2022 Nov; Vol. 111 (4), pp. 278-287. Date of Electronic Publication: 2022 Apr 27.
DOI:	10.1055/s-0041-1740967
Abstrakt:	Objective: The present study aimed to identify possible phenotypic changes in 4T1 (murine mammary adenocarcinoma) cells in vitro , including viability, HER-2 (human epidermal growth factor receptor-type 2) expression, and metastatic potential, after treatment with Carcinosinum in different homeopathic dilutions (12cH, 30cH, 200cH) shaken mechanically in pure, sterile, water from a commercial stock dilution. Methods: Treated cells were cultured in R10 medium, using 24-well plates, 10 5 cells per well, and treated with vehicle, Carcinosinum 12cH, 30cH or 200cH; untreated cells were used as the baseline control. After 24 hours of treatment, the percentage of apoptotic cells was analyzed by annexin V. Cell morphology was evaluated by microscopy after hematoxylin-eosin and Giemsa staining, whilst HER-2 expression was assessed using immunocytochemistry. The metastatic potential was determined by the expression and activity of the enzyme matrix metalloproteinase 9 (MMP-9) using zymography. The cytokine profile was established using the cytometric bead array method. Result: Treatment of 4T1 cells in vitro with Carcinosinum 30cH produced an increase in the number of annexin V-positive cells (apoptosis) and decreased expression of proactivated MMP-9. Cells treated with Carcinosinum 200cH presented hyper-expression of HER-2 on the plasma membrane, identified by immunocytochemistry. There were no differences in cytokine production among treatments. Conclusion: The data show promising results for Carcinosinum 30cH in vitro , but in vivo studies are also required to evaluate the role of tumor microenvironment in its effects. Competing Interests: None declared. (Faculty of Homeopathy. This article is published by Thieme.)
Databáze:	MEDLINE
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