Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi.

Autor: Anzibar Fialho M; Laboratorio de Acústica Ultrasonora, Instituto de Física, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay.; Física No Lineal, Instituto de Física de Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay., Vázquez Alberdi L; Laboratorio de Biología Celular del Sistema Nervioso Periférico, Departamento de Proteínas y Ácidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, 11600, Montevideo, Uruguay., Martínez M; Laboratorio de Biología Celular del Sistema Nervioso Periférico, Departamento de Proteínas y Ácidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, 11600, Montevideo, Uruguay., Calero M; Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, CIBERNED and CIEN Foundation, 28220, Madrid, Spain., Baranger J; Physics for Medicine Paris, Inserm U1273, ESPCI Paris, PSL University, CNRS UMR 8063, 75012, Paris, France., Tanter M; Physics for Medicine Paris, Inserm U1273, ESPCI Paris, PSL University, CNRS UMR 8063, 75012, Paris, France., Damián JP; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, 13000, Montevideo, Uruguay., Negreira C; Laboratorio de Acústica Ultrasonora, Instituto de Física, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay., Rubido N; Física No Lineal, Instituto de Física de Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay.; Institute for Complex Systems and Mathematical Biology, University of Aberdeen, King's College, Aberdeen, AB24 3UE, UK., Kun A; Laboratorio de Biología Celular del Sistema Nervioso Periférico, Departamento de Proteínas y Ácidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, 11600, Montevideo, Uruguay. akun@fcien.edu.uy.; Sección Bioquímica, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay. akun@fcien.edu.uy., Brum J; Laboratorio de Acústica Ultrasonora, Instituto de Física, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay. jbrum@fisica.edu.uy.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Apr 26; Vol. 12 (1), pp. 6784. Date of Electronic Publication: 2022 Apr 26.
DOI: 10.1038/s41598-022-10457-9
Abstrakt: The hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between blood flow and vascular structure-and how it changes with ageing-is physiologically and anatomically relevant. Ultrafast Doppler ([Formula: see text]Doppler) and scanning laser confocal microscopy (SLCM) are powerful imaging modalities that can measure in vivo cerebral blood volume (CBV) and post mortem vascular structure, respectively. Here, we apply both imaging modalities to a cross-sectional and longitudinal study of hippocampi vasculature in wild-type mice brains. We introduce a segmentation of CBV distribution obtained from [Formula: see text]Doppler and show that this mice-independent and mesoscopic measurement is correlated with vessel volume fraction (VVF) distribution obtained from SLCM-e.g., high CBV relates to specific vessel locations with large VVF. Moreover, we find significant changes in CBV distribution and vasculature due to ageing (5 vs. 21 month-old mice), highlighting the sensitivity of our approach. Overall, we are able to associate CBV with vascular structure-and track its longitudinal changes-at the artery-vein, venules, arteriole, and capillary levels. We believe that this combined approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes.
(© 2022. The Author(s).)
Databáze: MEDLINE
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