The effect of metformin on the metabolism of human vascular smooth muscle cells in high glucose conditions.

Autor:	Soleimani AA; Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran., Abkenar BR; Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran., Shokri N; Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran., Ghasempour G; Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran., Najafi M; Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Journal of basic and clinical physiology and pharmacology [J Basic Clin Physiol Pharmacol] 2022 Apr 28; Vol. 34 (1), pp. 55-59. Date of Electronic Publication: 2022 Apr 28 (Print Publication: 2023).
DOI:	10.1515/jbcpp-2022-0018
Abstrakt:	Objectives: Metformin is widely used in type 2 diabetic patients as an antihyperglycemic drug. The aim of this study was to investigate the effect of metformin on the metabolism of vascular smooth muscle cells in high glucose conditions. Materials and Methods: The vascular smooth muscle cells were cultured in DMEM F12 containing glucose as high as 25 mM. The preconditioned cells were then treated with metformin in doses of 1, 5, and 7 mM for 24 h. MTT method was used to determine cell viability. Biochemical parameters including lactate, glucose, total protein, creatinine, and triglyceride were measured in the cell culture after the treatment with metformin. Oil Red O staining method was used to stain the lipids in the cells. Results: Metformin reduced significantly (p<0.001) VSMC proliferation in a concentration-dependent manner. With the increase of glucose uptake by VSMCs, the cell lipid deposition was not changed. Other biochemical parameters such as lactate, triglyceride, total protein, and creatinine were significantly changed in the cell culture (p<0.05). Conclusions: Metformin increased the glucose uptake impacting metabolic pathways in VSMCs. It also increased the lactate efflux and protein metabolism without the change in cellular lipid deposition in high glucose conditions. (© 2022 Walter de Gruyter GmbH, Berlin/Boston.)
Databáze:	MEDLINE
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