Haploidy and aneuploidy in switchgrass mediated by misexpression of CENH3.
| Autor: | Yoon S; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA.; Dep. of Plant Sciences, Univ. of California, Davis, CA, USA., Bragg J; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Aucar-Yamato S; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Chanbusarakum L; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Dluge K; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Cheng P; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Blumwald E; Dep. of Plant Sciences, Univ. of California, Davis, CA, USA., Gu Y; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA., Tobias CM; USDA-ARS, Western Regional Research Laboratory, Albany, CA, USA. |
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| Jazyk: | angličtina |
| Zdroj: | The plant genome [Plant Genome] 2023 Jun; Vol. 16 (2), pp. e20209. Date of Electronic Publication: 2022 Apr 25. |
| DOI: | 10.1002/tpg2.20209 |
| Abstrakt: | Cross bred species such as switchgrass may benefit from advantageous breeding strategies requiring inbred lines. Doubled haploid production methods offer several ways that these lines can be produced that often involve uniparental genome elimination as the rate limiting step. We have used a centromere-mediated genome elimination strategy in which modified CENH3 is expressed to induce the process. Transgenic tetraploid switchgrass lines coexpressed Cas9, a poly-cistronic tRNA-gRNA tandem array containing eight guide RNAs that target two CENH3 genes, and different chimeric versions of CENH3 with alterations to the N-terminal tail region. Genotyping of CENH3 genes in transgenics identified edits including frameshift mutations and deletions in one or both copies of the two CENH3 genes. Flow cytometry of T (© 2022 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.) |
| Databáze: | MEDLINE |
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