Autologous Human Immunocompetent White Adipose Tissue-on-Chip.

Autor: Rogal J; Department for Microphysiological Systems, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany.; Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstr. 12, Stuttgart, 70569, Germany., Roosz J; NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, Reutlingen, 72770, Germany., Teufel C; Department for Microphysiological Systems, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany., Cipriano M; Department for Microphysiological Systems, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany.; 3R-Center for In vitro Models and Alternatives to Animal Testing, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany., Xu R; Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstr. 12, Stuttgart, 70569, Germany.; Harvard Medical School (HMS), 25 Shattuck St, Boston, MA, 02115, USA., Eisler W; Clinic for Plastic, Reconstructive, Hand and Burn Surgery, BG Trauma Center, Eberhard Karls University Tübingen, Schnarrenbergstraße 95, Tübingen, 72076, Germany., Weiss M; NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, Reutlingen, 72770, Germany.; Department of Women's Health, Eberhard Karls University Tübingen, Calwerstrasse 7, Tübingen, 72076, Germany., Schenke-Layland K; NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, Reutlingen, 72770, Germany.; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA, 90095, USA.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', Eberhard Karls University Tuebingen, Röntgenweg 11, Tuebingen, 72076, Germany.; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Silcherstr. 7/1, Tübingen, 72076, Germany., Loskill P; Department for Microphysiological Systems, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany.; NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, Reutlingen, 72770, Germany.; 3R-Center for In vitro Models and Alternatives to Animal Testing, Eberhard Karls University Tübingen, Österbergstr. 3, Tübingen, 72074, Germany.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2022 Jun; Vol. 9 (18), pp. e2104451. Date of Electronic Publication: 2022 Apr 24.
DOI: 10.1002/advs.202104451
Abstrakt: Obesity and associated diseases, such as diabetes, have reached epidemic proportions globally. In this era of "diabesity", white adipose tissue (WAT) has become a target of high interest for therapeutic strategies. To gain insights into mechanisms of adipose (patho-)physiology, researchers traditionally relied on animal models. Leveraging Organ-on-Chip technology, a microphysiological in vitro model of human WAT is introduced: a tailored microfluidic platform featuring vasculature-like perfusion that integrates 3D tissues comprising all major WAT-associated cellular components (mature adipocytes, organotypic endothelial barriers, stromovascular cells including adipose tissue macrophages) in an autologous manner and recapitulates pivotal WAT functions, such as energy storage and mobilization as well as endocrine and immunomodulatory activities. A precisely controllable bottom-up approach enables the generation of a multitude of replicates per donor circumventing inter-donor variability issues and paving the way for personalized medicine. Moreover, it allows to adjust the model's degree of complexity via a flexible mix-and-match approach. This WAT-on-Chip system constitutes the first human-based, autologous, and immunocompetent in vitro adipose tissue model that recapitulates almost full tissue heterogeneity and can become a powerful tool for human-relevant research in the field of metabolism and its associated diseases as well as for compound testing and personalized- and precision medicine applications.
(© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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