Microbial community origin and fate through a rural wastewater treatment plant.

Autor: Newton K; Institut de Recherche en Biologie Végétale, Université de Montréal, Montréal, H1X 2B2, Canada., Gonzalez E; Microbiome Unit, Canadian Center for Computational Genomics, Department of Human Genetics, McGill University, Montréal, H3A 1A4, Canada., Pitre FE; Institut de Recherche en Biologie Végétale, Université de Montréal, Montréal, H1X 2B2, Canada., Brereton NJB; Institut de Recherche en Biologie Végétale, Université de Montréal, Montréal, H1X 2B2, Canada.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2022 May; Vol. 24 (5), pp. 2516-2542. Date of Electronic Publication: 2022 May 04.
DOI: 10.1111/1462-2920.16025
Abstrakt: Conventional wastewater treatment relies on a complex microbiota; however, much of this community is still to be characterized. To better understand the origin, dynamics and fate of bacteria within a wastewater treatment plant: untreated primary wastewater, activated sludge and post-treatment effluent were characterized. From 3163 exact sequence variants (ESVs), 860 were annotated to species-level. In primary wastewater, 28% of ESVs were putative bacterial species previously associated with humans, 14% with animals and 5% as common to the environment. Differential abundance analysis revealed significant relative reductions in ESVs from potentially human-associated species from primary wastewater to activated sludge, and significant increases in ESVs from species associated with nutrient cycling. Between primary wastewater and effluent, 51% of ESVs from human-associated species did not significantly differ, and species such as Bacteroides massiliensis and Bacteroides dorei increased. These findings illustrate that activated sludge increased extracellular protease and urease-producing species, ammonia and nitrite oxidizers, denitrifiers and specific phosphorus accumulators. Although many human-associated species declined, some persisted in effluent, including strains of potential health or environmental concern. Species-level microbial assessment may be useful for understanding variation in wastewater treatment efficiency as well as for monitoring the release of microbes into surface water and the wider ecosystem.
(© 2022 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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