Metagenomic tracking of antibiotic resistance genes through a pre-harvest vegetable production system: an integrated lab-, microcosm- and greenhouse-scale analysis.

Autor: Keenum I; Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA., Wind L; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, USA., Ray P; Department of Animal Sciences, School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK., Guron G; Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, USA., Chen C; Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA, USA., Knowlton K; Department of Dairy Science, Virginia Tech, Blacksburg, VA, USA., Ponder M; Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, USA., Pruden A; Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2022 Aug; Vol. 24 (8), pp. 3705-3721. Date of Electronic Publication: 2022 May 18.
DOI: 10.1111/1462-2920.16022
Abstrakt: Prior research demonstrated the potential for agricultural production systems to contribute to the environmental spread of antibiotic resistance genes (ARGs). However, there is a need for integrated assessment of critical management points for minimizing this potential. Shotgun metagenomic sequencing data were analysed to comprehensively compare total ARG profiles characteristic of amendments (manure or compost) derived from either beef or dairy cattle (with and without dosing antibiotics according to conventional practice), soil (loamy sand or silty clay loam) and vegetable (lettuce or radish) samples collected across studies carried out at laboratory-, microcosm- and greenhouse-scale. Vegetables carried the greatest diversity of ARGs (n = 838) as well as the most ARG-mobile genetic element co-occurrences (n = 945). Radishes grown in manure- or compost-amended soils harboured a higher relative abundance of total (0.91 and 0.91 ARGs/16S rRNA gene) and clinically relevant ARGs than vegetables from other experimental conditions (average: 0.36 ARGs/16S rRNA gene). Lettuce carried the highest relative abundance of pathogen gene markers among the metagenomes examined. Total ARG relative abundances were highest on vegetables grown in loamy sand receiving antibiotic-treated beef amendments. The findings emphasize that additional barriers, such as post-harvest processes, merit further study to minimize potential exposure to consumers.
(© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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