Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

Autor:	Visscher M; Department of Cardiology, Erasmus MC University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands., Pleitez MA; Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.; Chair of Biological Imaging (CBI) and Center for Translational Cancer Research (TranslaTUM), Technische Universität München, München, Germany., Van Gaalen K; Department of Cardiology, Erasmus MC University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands., Nieuwenhuizen-Bakker IM; Department of Cardiology, Erasmus MC University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands., Ntziachristos V; Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.; Chair of Biological Imaging (CBI) and Center for Translational Cancer Research (TranslaTUM), Technische Universität München, München, Germany., Van Soest G; Department of Cardiology, Erasmus MC University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands.
Jazyk:	angličtina
Zdroj:	Photoacoustics [Photoacoustics] 2022 Apr 11; Vol. 26, pp. 100354. Date of Electronic Publication: 2022 Apr 11 (Print Publication: 2022).
DOI:	10.1016/j.pacs.2022.100354
Abstrakt:	Background and Aims: Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth. Methods: We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF). Results: We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively. Conclusions: These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
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