Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models.
Autor: | Alsareii SA; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Alzerwi NAN; Department of Surgery, College of Medicine, Majmaah University, Ministry of Education, Al-Majmaah City, Saudi Arabia., AlAsmari MY; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Alamri AM; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Mahnashi MH; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia., Shaikh IA; Department of Pharmacology, College of Pharmacy, Najran University, Najran, Saudi Arabia. |
---|---|
Jazyk: | angličtina |
Zdroj: | Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2022 Apr 13; Vol. 2022, pp. 6449550. Date of Electronic Publication: 2022 Apr 13 (Print Publication: 2022). |
DOI: | 10.1155/2022/6449550 |
Abstrakt: | Background: When the skin and tissues within the body are injured, the healing process begins. Medicinal herbs have been used to cure wounds since time immemorial. The antimicrobial and antioxidant activity possessed by P. integrifolia may accelerate wound healing. Objectives: To assess the wound healing activity of Premna integrifolia extract (PIE) by employing in-vivo experimental animal models and an in-vitro migration scratch assay. Furthermore, to assess its cytotoxicity using the MTT assay. Methods: Wistar albino rats were used for the in vivo wound healing models. The animals were divided into four groups at random: Group I was untreated. Group II was vehicle control (ointment base). Group III was PIE ointment (5% W/W). Group IV was standard (povidone-iodine ointment) (5% W/W). The ointments were applied directly to the wounds as described above until they healed completely. The wound contraction percentage and tensile strength were calculated. The MTT test was used to determine the viability of the test extract against the fibroblast cells. The scratch assay was used in vitro to determine the wound healing potential of the test drug. P ≤ 0.05 values were considered statistically significant. Results: Premna integrifolia extract did not possess any noticeable cytotoxicity to the cell line and showed an IC Conclusion: The current study found that Premna integrifolia improved wound healing activity both in vitro and in vivo . These findings indicate that Premna integrifolia extract has wound-healing potential and could be a viable source of nutraceuticals with wound-healing properties. Competing Interests: The authors declare no conflicts of interest. (Copyright © 2022 Saeed Ali Alsareii et al.) |
Databáze: | MEDLINE |
Externí odkaz: |