Microarray dataset supporting a role for ATF4 in isoginkgetin-induced gene expression in HCT116 cells.
| Autor: | van Zyl E; Department of Biology, Carleton University., Tolls V; Department of Biology, Carleton University., McKay BC; Department of Biology, Carleton University.; Institute of Biochemistry, Carleton University. |
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| Jazyk: | angličtina |
| Zdroj: | Data in brief [Data Brief] 2022 Apr 01; Vol. 42, pp. 108126. Date of Electronic Publication: 2022 Apr 01 (Print Publication: 2022). |
| DOI: | 10.1016/j.dib.2022.108126 |
| Abstrakt: | Isoginkgetin (IGG) is a compound originally derived from the leaves of Ginkgo biloba trees. It was subsequently identified through a chemical screen to be an inhibitor of both the major and minor spliceosome, with an IC50 value of 30 µM [1]. Little is currently known about the overall effects of spliceosome inhibition on human cells. Here, we treated HCT116 and a p53 null subline of colon cancer cells with 30 µM IGG for 8 hours. Total RNA was isolated, and Affymetrix oligonucleotide microarray analysis was completed using samples from two biologically independent experiments. A relatively small number of transcripts were differentially expressed in these cell lines. There was considerable overlap in the upregulated but not the downregulated transcripts. PANTHER Reactome analysis of these shared upregulated transcripts identified enriched pathways involving the ATF4 transcription factor important in the integrated stress response [2]. Competing Interests: The authors have no competing interests to declare (© 2022 The Authors. Published by Elsevier Inc.) |
| Databáze: | MEDLINE |
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