Integrative analysis of metabolome and transcriptome provide new insights into the bitter components of Lilium lancifolium and Lilium brownii.

Autor: Qin Y; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao Special Administrative Region of China., Jin J; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Zhou RR; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Fang LZ; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Liu H; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Zhong C; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Xie Y; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Liu PA; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China., Qin YH; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China. Electronic address: dlqyh@sohu.com., Zhang SH; Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410013, China. Electronic address: zhangshuihan0220@126.com.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2022 Jun 05; Vol. 215, pp. 114778. Date of Electronic Publication: 2022 Apr 20.
DOI: 10.1016/j.jpba.2022.114778
Abstrakt: Lilium, a perennial crop with great ornamental, medicinal and edible value, has been frequently used as functional food and medicine. Lilium lancifolium Thunb. (L. lancifolium) and Lilium brownii F.E.Brown var.viridulum Baker (L. brownii) are the most used medicinal species in China. However, the flavor compounds of these two species have not yet been clear. Here, metabolomics and transcriptome analysis were used to reveal the difference of the bitter substances of L. lancifolium and L. brownii. Qualitative results indicated that nine compounds are commonly existed in L. lancifolium and L. brownii, while nine compounds are unique in L. lancifolium and eight compounds are unique in L. brownii. Furthermore, quantitative results revealed that the content of regaloside A in L. lancifolium was nearly 2-7 folds higher than that of L. brownii, and the content of regaloside B in L. lancifolium was about 4-16 folds higher than that of L. brownii. Regaloside C and E were not detected in L. brownii. Transcriptome analysis showed that there were 90 unique genes up-regulated in L. lancifolium samples in the pathway of phenylpropanoid biosynthesis and 75 unique genes up-regulated in L. brownii samples, which could be related to the different content and chemical structure specificity of phenylpropanoid glycerol glucosides in L. lancifolium and L. brownii. The results of our in-deep research provide new insights into the bitter substances of L. lancifolium and L. brownii, and a further consideration for the chemical consistency and quality evaluation for Lilii bulbus.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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