Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling.

Autor:	Aboobucker SI; Department of Agronomy, Iowa State University, Ames, IA, USA. siddique@iastate.edu., Jubery TZ; Department of Mechanical Engineering, Iowa State University, Ames, IA, USA., Frei UK; Department of Agronomy, Iowa State University, Ames, IA, USA., Chen YR; Department of Agronomy, Iowa State University, Ames, IA, USA., Foster T; Department of Agronomy, Iowa State University, Ames, IA, USA., Ganapathysubramanian B; Department of Mechanical Engineering, Iowa State University, Ames, IA, USA., Lübberstedt T; Department of Agronomy, Iowa State University, Ames, IA, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2484, pp. 213-235.
DOI:	10.1007/978-1-0716-2253-7_16
Abstrakt:	Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here. The major steps involved in in vivo DH technology are haploid induction, haploid selection/identification, and haploid genome doubling. Herein, we elaborate on the various steps of DH technology in maize breeding from haploid induction to haploid genome doubling to produce DH lines. Detailed protocols on the following topics are discussed: in vivo haploid inducer line development, haploid selection using seed and root color markers and automated seed sorting based on embryo oil content using QSorter, artificial genome doubling, and the identification of genotypes with spontaneous haploid genome doubling (SHGD) ability. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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