Generation of Mutant Plants by Gamma Ray Exposure and Development of Low-Cost TILLING Population in Chickpea (Cicer arietinum L.).
| Autor: | Amri-Tiliouine W; Laboratory of Integrative Improvement of Vegetal Productions, Higher National Agronomic School (ENSA), Algiers, Algeria.; Division of Biotechnology and Plant Breeding, National Institute of Agricultural Research of Algeria (I.N.R.A.A.), Algiers, Algeria., Jankowicz-Cieslak J; Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf, International Atomic Energy Agency, Vienna, Austria., Till BJ; Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf, International Atomic Energy Agency, Vienna, Austria.; Veterinary Genetics Laboratory, University of California, Davis, Davis, CA, USA., Laouar M; Laboratory of Integrative Improvement of Vegetal Productions, Higher National Agronomic School (ENSA), Algiers, Algeria. m.laouar@ensa.dz. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2484, pp. 143-159. |
| DOI: | 10.1007/978-1-0716-2253-7_12 |
| Abstrakt: | Induced mutations have been used to facilitate plant breeding for more than 80 years. Success requires the development of a mutant population and methods to evaluate that population. In this protocol we provide methods for the development of a chickpea mutant population using gamma irradiation, and low-cost methods for the molecular characterization of the mutant population. Specifically, this chapter provides detailed methods for (1) mutation induction by gamma rays and determination of LD (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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