A flavonoid-rich fraction of Monolluma quadrangula inhibits xanthine oxidase and ameliorates potassium oxonate-induced hyperuricemia in rats.

Autor: ALRashdi BM; Biology Department, College of Science, Jouf University, Sakakah, Saudi Arabia., Elgebaly HA; Biology Department, College of Science, Jouf University, Sakakah, Saudi Arabia., Germoush MO; Biology Department, College of Science, Jouf University, Sakakah, Saudi Arabia., Qarmush MM; College of Medicine, Jouf University, Sakakah, Saudi Arabia., Azab MS; Biology Department, College of Science, Jouf University, Sakakah, Saudi Arabia.; Zoology Department, Faculty of Science, Benha University, Benha, Egypt., Alruhaimi RS; Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia., Ahmeda AF; Department of Basic Medical Sciences, College of Medicine, Ajman University, Ajman, United Arab Emirates.; Center of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates., Abukhalil MH; Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, Jordan., Kamel EM; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Arab HH; Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif, Saudi Arabia., Alzoghaibi MA; Physiology Department, College of Medicine, King Saud University, Riyadh, Saudi Arabia., Alotaibi MF; Physiology Department, College of Medicine, King Saud University, Riyadh, Saudi Arabia., Mahmoud AM; Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Salah Salim St, Beni-Suef, 62514, Egypt. ayman.mahmoud@science.bsu.edu.eg.; Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Manchester, UK. ayman.mahmoud@science.bsu.edu.eg.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Sep; Vol. 29 (42), pp. 63520-63532. Date of Electronic Publication: 2022 Apr 23.
DOI: 10.1007/s11356-022-20274-2
Abstrakt: Hyperuricemia represents a risk factor for the progression of chronic kidney disease. Oxidative stress and inflammation are implicated in the mechanisms underlying hyperuricemia-mediated kidney injury. Monolluma quadrangula possesses several beneficial effects; however, its effect on hyperuricemia has not been investigated. This study evaluated the renoprotective and xanthine oxidase (XO) inhibitory activity of M. quadrangula in hyperuricemic rats. Phytochemical investigation revealed the presence of six known flavonoid isolated for the first time from this species. The rats received M. quadrangula extract (MQE) and potassium oxonate (PO) for 7 days. In vitro assays showed the radical scavenging and XO inhibitory activities of MQE, and in silico molecular docking revealed the inhibitory activity of the isolated flavonoids towards XO. Hyperuricemic rats showed elevated serum uric acid, creatinine, urea, and XO activity, and renal pro-inflammatory cytokines, MDA and NO, and decreased GSH, SOD, and catalase. MQE ameliorated serum uric acid, urea, creatinine, and XO activity, and renal pro-inflammatory cytokines. In addition, MQE attenuated renal oxidative stress, enhanced antioxidants, downregulated URAT-1, and GLUT-9 and upregulated OAT-1 in PO-induced rats. In conclusion, M. quadrangula attenuated hyperuricemia and kidney impairment by suppressing XO activity, oxidative stress and inflammation, and modulating urate transporters.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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