Whole-exome sequencing reveals damaging gene variants associated with hypoalphalipoproteinemia.

Autor:	Dong W; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA., Wong KHY; Cardiovascular Research Institute, University of California, San Francisco, CA, USA., Liu Y; Department of Cardiology, The Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China., Levy-Sakin M; Cardiovascular Research Institute, University of California, San Francisco, CA, USA., Hung WC; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA., Li M; Department of Biostatistics, Yale School of Public Health, New Haven, CT, USA., Li B; Department of Biostatistics, Yale School of Public Health, New Haven, CT, USA., Jin SC; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA; Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA., Choi J; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Korea., Lopez-Giraldez F; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA., Vaka D; Institute for Human Genetics, University of California, San Francisco, CA, USA., Poon A; Institute for Human Genetics, University of California, San Francisco, CA, USA., Chu C; Institute for Human Genetics, University of California, San Francisco, CA, USA., Lao R; Institute for Human Genetics, University of California, San Francisco, CA, USA., Balamir M; Department of Internal Medicine, Istanbul University, Istanbul, Turkey., Movsesyan I; Cardiovascular Research Institute, University of California, San Francisco, CA, USA., Malloy MJ; Cardiovascular Research Institute, University of California, San Francisco, CA, USA; Department of Medicine, University of California, San Francisco, CA, USA; Department of Pediatrics, University of California, San Francisco, CA, USA., Zhao H; Department of Biostatistics, Yale School of Public Health, New Haven, CT, USA., Kwok PY; Cardiovascular Research Institute, University of California, San Francisco, CA, USA; Department of Medicine, University of California, San Francisco, CA, USA; Department of Dermatology, University of California, San Francisco, CA, USA., Kane JP; Cardiovascular Research Institute, University of California, San Francisco, CA, USA; Department of Medicine, University of California, San Francisco, CA, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA., Lifton RP; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA., Pullinger CR; Cardiovascular Research Institute, University of California, San Francisco, CA, USA; Physiological Nursing, University of California, San Francisco, CA, USA. Electronic address: clive.pullinger@ucsf.edu.
Jazyk:	angličtina
Zdroj:	Journal of lipid research [J Lipid Res] 2022 Jun; Vol. 63 (6), pp. 100209. Date of Electronic Publication: 2022 Apr 20.
DOI:	10.1016/j.jlr.2022.100209
Abstrakt:	Low levels of high density lipoprotein-cholesterol (HDL-C) are associated with an elevated risk of arteriosclerotic coronary heart disease. Heritability of HDL-C levels is high. In this research discovery study, we used whole-exome sequencing to identify damaging gene variants that may play significant roles in determining HDL-C levels. We studied 204 individuals with a mean HDL-C level of 27.8 ± 6.4 mg/dl (range: 4-36 mg/dl). Data were analyzed by statistical gene burden testing and by filtering against candidate gene lists. We found 120 occurrences of probably damaging variants (116 heterozygous; four homozygous) among 45 of 104 recognized HDL candidate genes. Those with the highest prevalence of damaging variants were ABCA1 (n = 20), STAB1 (n = 9), OSBPL1A (n = 8), CPS1 (n = 8), CD36 (n = 7), LRP1 (n = 6), ABCA8 (n = 6), GOT2 (n = 5), AMPD3 (n = 5), WWOX (n = 4), and IRS1 (n = 4). Binomial analysis for damaging missense or loss-of-function variants identified the ABCA1 and LDLR genes at genome-wide significance. In conclusion, whole-exome sequencing of individuals with low HDL-C showed the burden of damaging rare variants in the ABCA1 and LDLR genes is particularly high and revealed numerous occurrences in HDL candidate genes, including many genes identified in genome-wide association study reports. Many of these genes are involved in cancer biology, which accords with epidemiologic findings of the association of HDL deficiency with increased risk of cancer, thus presenting a new area of interest in HDL genomics. Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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