Preliminary findings of age and male sexual characteristics andand potential effect to semen characteristics and cryopreservation of the critically endangered Bornean orangutan in Malaysia.

Autor: Zainuddin ZZ; Bringing Back Our Rare Animal, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu, 88400, Sabah, Malaysia., Tarmizi R; Bringing Back Our Rare Animal, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu, 88400, Sabah, Malaysia., Chee YK; Bringing Back Our Rare Animal, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu, 88400, Sabah, Malaysia., Sarkawi NN; Sabah Wildlife Department, Level 4, Block B, Wisma MUIS, Kota Kinabalu, 88100, Sabah, Malaysia., Ahmad H; Department of Veterinary Preclinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Salleh A; Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Tahir NDM; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Baiee F; Department of Clinical Science, Faculty of Veterinary Medicine, University of Kufa, 54003, Najaf, Iraq., Che-Amat A; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Fitri WN; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. wannorfitri@upm.edu.my.
Jazyk: angličtina
Zdroj: Primates; journal of primatology [Primates] 2022 Jul; Vol. 63 (4), pp. 377-386. Date of Electronic Publication: 2022 Apr 23.
DOI: 10.1007/s10329-022-00989-z
Abstrakt: Bornean orangutan is a critically endangered non-human primate; however, the threat of extinction is not merely from poaching and habitat loss. Orangutan survival is also threatened by the genetic loss and genetic bottleneck due to the low effective population, prompting the dire need for an immediate genetic preservation program through systematic biobanking and assisted reproductive technology (ART). This study aims to provide integral data to the semen characteristics, extension, and cryopreservation of the Bornean orangutan and the potential relationship to male traits. Five captive orangutans from Sepilok Orangutan Rehabilitation Centre (SORC) with a mean body weight of 52.81 ± 7.00 kg were used for this study. Semen collection was performed using electroejaculation (EE) under complete general anesthesia. Semen was subjected to macroscopic and microscopic evaluation while testicular measurement was obtained using digital calipers. The semen characteristics of the orangutans are volume (778 ± 250.21 µl), pH (7.80 ± 0.25), concentration (32.38 ± 17.40 × 10 6 sperm/ml), total motility (61.00 ± 12.88%), adjusted motility index (48.76 ± 11.32%), live spermatozoa (77.75 ± 6.94%) and normal spermatozoa (11.48 ± 11.34%). Analysis of variance statistical analysis test was used to compare the significant difference between means, at (p < 0.05). Spermatozoa concentration was the only significant different parameter between individuals. Testes biometry parameters are statistically significant between the flanged and unflanged individuals. Live spermatozoa are different in adult and sub-adult individual while teratospermia was found to be consistently high in all individuals. Chilled and post-thaw quality after cryopreservation suggests promising survivability of spermatozoa. Semen collection with EE yields a consistent and acceptable quality of spermatozoa for cryopreservation, biobanking purposes, and potential application of ART.
(© 2022. The Author(s), under exclusive licence to Japan Monkey Centre.)
Databáze: MEDLINE
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