Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer.

Autor: Bartha L; Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babeş-Bolyai University, 400271, Cluj-Napoca, Romania., Mandáková T; Central European Institute of Technology (CEITEC), Masaryk University, 625 00, Brno, Czech Republic., Kovařík A; Laboratory of Molecular Epigenetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, 61265, Brno, Czech Republic., Bulzu PA; Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre of the Academy of Sciences of the Czech Republic, 370 05, České Budějovice, Czech Republic., Rodde N; French Plant Genomic Resource Center, INRAE-CNRGV, 31320, Castanet Tolosan, France., Mahelka V; Institute of Botany, Czech Academy of Sciences, 25243, Průhonice, Czech Republic., Lysak MA; Central European Institute of Technology (CEITEC), Masaryk University, 625 00, Brno, Czech Republic., Fustier MA; French Plant Genomic Resource Center, INRAE-CNRGV, 31320, Castanet Tolosan, France., Šafář J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, 779 00, Olomouc, Czech Republic., Cápal P; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, 779 00, Olomouc, Czech Republic., Keresztes L; Hungarian Department of Biology and Ecology, Babeş-Bolyai University, 400006, Cluj-Napoca, Romania.; Centre of Systems Biology, Biodiversity and Bioresources (3B), Babeş-Bolyai University, 400006, Cluj-Napoca, Romania., Banciu HL; Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babeş-Bolyai University, 400271, Cluj-Napoca, Romania.; Centre of Systems Biology, Biodiversity and Bioresources (3B), Babeş-Bolyai University, 400006, Cluj-Napoca, Romania.; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 400006, Cluj-Napoca, Romania.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2022 Aug; Vol. 235 (3), pp. 1246-1259. Date of Electronic Publication: 2022 May 23.
DOI: 10.1111/nph.18171
Abstrakt: During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot-type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens-canis. This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. In this work we aimed to support this hypothesis by carrying out genomic, molecular, and cytogenetic analyses. Genome skimming coupled by PacBio HiFi sequencing of a bacterial artificial chromosome clone derived from flow-sorted nuclei was used to characterise the alien 45S rDNA. Integration of alien rDNA in the recipient genome was further proved by Southern blotting and fluorescence in situ hybridization using specific probes. Alien rDNA, nested among Potentilla species in phylogenetic analysis, likely entered the Erythronium lineage in the common ancestor of E. dens-canis and E. caucasicum. Transferred eudicot-type rDNA preserved its tandemly arrayed feature on a single chromosome and was found to be transcribed in the monocot host, albeit much less efficiently than the native counterpart. This study adds a new example to the rarely documented nuclear-to-nuclear jumps of DNA between eudicots and monocots while holding the scientific community continually in suspense about the mode of DNA transfer.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
Databáze: MEDLINE
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