Maternal breast milk microbiota and immune markers in relation to subsequent development of celiac disease in offspring.

Autor: Štšepetova J; Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila Street 19, 50411, Tartu, Estonia. jelena.stsepetova@ut.ee., Simre K; Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411, Tartu, Estonia. kart.simre@kliinikum.ee.; Children's Clinic, Tartu University Hospital, Lunini 6, 50406, Tartu, Estonia. kart.simre@kliinikum.ee., Tagoma A; Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411, Tartu, Estonia., Uibo O; Children's Clinic, Tartu University Hospital, Lunini 6, 50406, Tartu, Estonia.; Department of Pediatrics, Institute of Clinical Medicine, University of Tartu, Lunini 6, 50406, Tartu, Estonia., Peet A; Children's Clinic, Tartu University Hospital, Lunini 6, 50406, Tartu, Estonia.; Department of Pediatrics, Institute of Clinical Medicine, University of Tartu, Lunini 6, 50406, Tartu, Estonia., Siljander H; Pediatric Research Center, Children's Hospital, University of Helsinki, Helsinki University Hospital, Stenbäckinkatu 9, PO Box 347, Helsinki, Finland.; Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Biomedicum 1, Haartmaninkatu 8, 00290, Helsinki, Finland.; Center of Military Medicine, Finnish Defence Forces Logistics Command, Tampere, Finland., Tillmann V; Children's Clinic, Tartu University Hospital, Lunini 6, 50406, Tartu, Estonia.; Department of Pediatrics, Institute of Clinical Medicine, University of Tartu, Lunini 6, 50406, Tartu, Estonia., Knip M; Pediatric Research Center, Children's Hospital, University of Helsinki, Helsinki University Hospital, Stenbäckinkatu 9, PO Box 347, Helsinki, Finland.; Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Biomedicum 1, Haartmaninkatu 8, 00290, Helsinki, Finland.; Center for Child Health Research, Tampere University Hospital, Teiskontie 35, 33520, Tampere, Finland., Mändar R; Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila Street 19, 50411, Tartu, Estonia.; Competence Center on Health Technologies, Teaduspargi 13, 50411, Tartu, Estonia., Uibo R; Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411, Tartu, Estonia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Apr 22; Vol. 12 (1), pp. 6607. Date of Electronic Publication: 2022 Apr 22.
DOI: 10.1038/s41598-022-10679-x
Abstrakt: The potential impact of the composition of maternal breast milk is poorly known in children who develop celiac disease (CD). The aim of our study was to compare the microbiota composition and the concentrations of immune markers in breast milk from mothers whose offspring carried the genetic predisposition to CD, and whether they did or did not develop CD during follow-up for the first 3 years of life. Maternal breast milk samples [CD children (n = 6) and healthy children (n = 18)] were collected 3 months after delivery. Enzyme-linked immunosorbent assays were used to measure TGF-β1, TGF-β2, sIgA, MFG-E8 and sCD14. For microbiota analysis, next generation (Illumina) sequencing, real-time PCR and denaturing gradient gel electrophoresis were used. Phylotype abundance and the Shannon 'H' diversity index were significantly higher in breast milk samples in the CD group. There was higher prevalence of the phyla Bacteroidetes and Fusobacteria, the classes Clostridia and Fusobacteriia, and the genera Leptotrichia, Anaerococcus, Sphingomonas, Actynomyces and Akkermansia in the CD group. The immunological markers were differently associated with some Gram-negative bacterial genera and species (Chryseobacterium, Sphingobium) as well as Gram-positive species (Lactobacillus reuteri, Bifidobacterium animalis). In conclusion, the microbiota in breast milk from mothers of genetically predisposed offspring who presented CD showed a higher bacterial phylotype abundance and diversity, as well as a different bacterial composition, as compared with the mothers of unaffected offspring. These immune markers showed some associations with bacterial composition and may influence the risk for development of CD beyond early childhood.
(© 2022. The Author(s).)
Databáze: MEDLINE
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