| Autor: |
Kiang NY; NASA Goddard Institute for Space Studies, New York, NY 10025, USA.; Virtual Planetary Laboratory, Nexus for Exoplanet System Science, NASA Astrobiology Program, USA., Swingley WD; Virtual Planetary Laboratory, Nexus for Exoplanet System Science, NASA Astrobiology Program, USA.; Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA., Gautam D; Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA., Broddrick JT; NASA Ames Research Center, Moffett Field, CA 94035, USA., Repeta DJ; Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA., Stolz JF; Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA.; Center for Environmental Research and Education, Duquesne University, Pittsburgh, PA 15282, USA., Blankenship RE; Departments of Biology and Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA., Wolf BM; Departments of Biology and Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA., Detweiler AM; NASA Ames Research Center, Moffett Field, CA 94035, USA.; Bay Area Environmental Research Institute, Moffett Field, CA 94035, USA., Miller KA; University Herbarium, University of California, Berkeley, CA 94720, USA., Schladweiler JJ; Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA., Lindeman R; Citizen Scientist, Alameda, CA 94501, USA., Parenteau MN; Virtual Planetary Laboratory, Nexus for Exoplanet System Science, NASA Astrobiology Program, USA.; NASA Ames Research Center, Moffett Field, CA 94035, USA. |
| Abstrakt: |
We have isolated a chlorophyll- d -containing cyanobacterium from the intertidal field site at Moss Beach, on the coast of Central California, USA, where Manning and Strain (1943) originally discovered this far-red chlorophyll. Here, we present the cyanobacterium's environmental description, culturing procedure, pigment composition, ultrastructure, and full genome sequence. Among cultures of far-red cyanobacteria obtained from red algae from the same site, this strain was an epiphyte on a brown macroalgae. Its Q y in vivo absorbance peak is centered at 704-705 nm, the shortest wavelength observed thus far among the various known Acaryochloris strains. Its Chl a /Chl d ratio was 0.01, with Chl d accounting for 99% of the total Chl d and Chl a mass. TEM imagery indicates the absence of phycobilisomes, corroborated by both pigment spectra and genome analysis. The Moss Beach strain codes for only a single set of genes for producing allophycocyanin. Genomic sequencing yielded a 7.25 Mbp circular chromosome and 10 circular plasmids ranging from 16 kbp to 394 kbp. We have determined that this strain shares high similarity with strain S15, an epiphyte of red algae, while its distinct gene complement and ecological niche suggest that this strain could be the closest known relative to the original Chl d source of Manning and Strain (1943). The Moss Beach strain is designated Acaryochloris sp. ( marina ) strain Moss Beach. |
