Identification and Subtyping of Salmonella Isolates Using Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF).

Autor: Persad AK; Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.; School of Veterinary Medicine, Faculty of Medical Sciences, The University of the West Indies, Eric Williams Medical Sciences Complex, Mt. Hope, Trinidad and Tobago, West Indies., Fahmy HA; Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.; Animal Health Research Institute (AHRI), Giza 12618, Egypt., Anderson N; Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA., Cui J; Animal Disease Diagnostic Laboratory, Ohio Department of Agriculture, 8995 East Main St., Building 6, Reynoldsburg, OH 43068, USA., Topalcengiz Z; Department of Food Engineering, Faculty of Engineering and Architecture, Muş Alparslan University, Muş 49250, Turkey.; Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA., Jeamsripong S; Western Institute for Food Safety and Security, UC Davis, Davis, CA 95618, USA.; Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand., Spanninger PM; Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA., Buchanan RL; Department of Nutrition and Food Science and Center for Food Safety and Security Systems, University of Maryland, College Park, MA 20742, USA., Kniel KE; Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA., Jay-Russell MT; Western Institute for Food Safety and Security, UC Davis, Davis, CA 95618, USA., Danyluk MD; Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA., Rajashekara G; Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA., LeJeune JT; Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.
Jazyk: angličtina
Zdroj: Microorganisms [Microorganisms] 2022 Mar 23; Vol. 10 (4). Date of Electronic Publication: 2022 Mar 23.
DOI: 10.3390/microorganisms10040688
Abstrakt: Subtyping of bacterial isolates of the same genus and species is an important tool in epidemiological investigations. A number of phenotypic and genotypic subtyping methods are available; however, most of these methods are labor-intensive and time-consuming and require considerable operator skill and a wealth of reagents. Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF), an alternative to conventional subtyping methods, offers a rapid, reproducible method for bacterial identification with a high sensitivity and specificity and at minimal cost. The purpose of this study was to determine the feasibility of using MALDI-TOF to differentiate between six Salmonella serovars recovered from experimental microcosms inoculated with known strains of Salmonella . Following the establishment of a MALDI-TOF reference library for this project, the identity of 843 Salmonella isolates recovered from these microcosms was assessed using both MALDI-TOF and conventional methods (serotyping/PCR). All 843 isolates were identified as being Salmonella species. Overall, 803/843 (95%) of these isolates were identified similarly using the two different methods. Positive percent agreement at the serovar level ranged from 79 to 100%, and negative percent agreement for all serovars was greater than 98%. Cohen's kappa ranged from 0.85 to 0.98 for the different serovars. This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Databáze: MEDLINE