| Autor: |
de Lima LF; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., Andrade-Pinheiro JC; Bioassay Laboratory (Labio), Federal University of Cariri (UFCA), Brejo Santo 63260-000, Brazil., Freitas MA; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, Brazil., da Silva AI; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, Brazil., Fonseca VJA; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., da Silva TG; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., da Silva JCP; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., de Lima RH; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., Sales DL; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil., Neves RP; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, Brazil., de Brito ES; Laboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, Brazil., Ribeiro PRV; Laboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, Brazil., Canuto KM; Laboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, Brazil., Coutinho HDM; Laboratory of Microbiology and Molecular Biology (LMBM), University Regional of Cariri (URCA), Crato 63105-010, Brazil., Siyadatpanah A; Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand 9717853577, Iran., Kim B; Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea., Morais-Braga MFB; Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil. |
| Abstrakt: |
(1) Background: Candida is a genus of yeasts with notable pathogenicity and significant ability to develop antimicrobial resistance. Gossypium hirsutum L., a medicinal plant that is traditionally used due to its antimicrobial properties, has demonstrated significant antifungal activity. Therefore, this study investigated the chemical composition and anti- Candida effects of aqueous (AELG) and hydroethanolic (HELG) extracts obtained from the leaves of this plant. (2) Methods: The extracts were chemically characterized by UPLC-QTOF-MS/MS, and their anti- Candida activities were investigated by analyzing cell viability, biofilm production, morphological transition, and enhancement of antifungal resistance. (3) Results: The UPLC-QTOF-MS/MS analysis revealed the presence of twenty-one compounds in both AELG and HELG, highlighting the predominance of flavonoids. The combination of the extracts with fluconazole significantly reduced its IC 50 values against Candida albicans INCQS 40006, Candida tropicalis INCQS 40042, and C. tropicalis URM 4262 strains, indicating enhanced antifungal activity. About biofilm production, significant inhibition was observed only for the AELG-treated C. tropicalis URM 4262 strain in comparison with the untreated control. Accordingly, this extract showed more significant inhibitory effects on the morphological transition of the INCQS 40006 and URM 4387 strains of C. albicans (4) Conclusions: Gossypium hirsutum L. presents promising antifungal effects, that may be potentially linked to the combined activity of chemical constituents identified in its extracts. |
