Connexin Expression in Pituitary Adenomas and the Effects of Overexpression of Connexin 43 in Pituitary Tumor Cell Lines.

Autor:	Nunes B; Laboratory of Experimental Endocrinology-LEEx, Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Postgraduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Laboratory of Endocrine Physiology, Doris Rosenthal, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Pópulo H; Institute for Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)-Cancer Signalling & Metabolism, 4200-135 Porto, Portugal.; Department of Pathology, Medical Faculty of the University of Porto, 4200-319 Porto, Portugal., Lopes JM; Institute for Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)-Cancer Signalling & Metabolism, 4200-135 Porto, Portugal.; Department of Pathology, Medical Faculty of the University of Porto, 4200-319 Porto, Portugal., Reis M; Institute for Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)-Cancer Signalling & Metabolism, 4200-135 Porto, Portugal., Nascimento G; Centre of Clinical Research (CEPEC), President Dutra Hospital of Federal University of Maranhão (UFMA), São Luís 65020-600, Brazil.; Endocrinology Service, President Dutra Hospital of Federal University of Maranhão (UFMA), São Luís 65060-600, Brazil., Nascimento AG; Pathology Service, President Dutra Hospital of Federal University of Maranhão (UFMA), São Luís 65020-070, Brazil., Fernandes J; NUPEX, Polo Duque de Caxias, Universidade Federal do Rio de Janeiro, Rio de Janeiro 25240-005, Brazil., Faria M; Centre of Clinical Research (CEPEC), President Dutra Hospital of Federal University of Maranhão (UFMA), São Luís 65020-600, Brazil.; Endocrinology Service, President Dutra Hospital of Federal University of Maranhão (UFMA), São Luís 65060-600, Brazil., de Carvalho DP; Laboratory of Experimental Endocrinology-LEEx, Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Postgraduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Laboratory of Endocrine Physiology, Doris Rosenthal, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Soares P; Institute for Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)-Cancer Signalling & Metabolism, 4200-135 Porto, Portugal.; Department of Pathology, Medical Faculty of the University of Porto, 4200-319 Porto, Portugal., Miranda-Alves L; Laboratory of Experimental Endocrinology-LEEx, Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Postgraduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Laboratory of Endocrine Physiology, Doris Rosenthal, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Postgraduate Program in Pharmacology and Medicinal Chemistry, Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2022 Apr 12; Vol. 13 (4). Date of Electronic Publication: 2022 Apr 12.
DOI:	10.3390/genes13040674
Abstrakt:	Gap junction intercellular communication (GJIC) is considered a key mechanism in the regulation of tissue homeostasis. GJIC structures are organized in two transmembrane channels, with each channel formed by connexins (Cxs). GJIC and Cxs expression alterations are related to the process of tumorigenesis in different cell types. Pituitary neuroendocrine tumors (PitNETs) represent 15-20% of intracranial neoplasms, and usually display benign behavior. Nevertheless, some may have aggressive behavior, invading adjacent tissues, and featuring a high proliferation rate. We aimed to assess the expression and relevance of GJIC and Cxs proteins in PitNETs. We evaluated the mRNA expression levels of Cx26, 32, and 43, and the protein expression of Cx43 in a series of PitNETs. In addition, we overexpressed Cx43 in pituitary tumor cell lines. At the mRNA level, we observed variable expression of all the connexins in the tumor samples. Cx43 protein expression was absent in most of the pituitary tumor samples that were studied. Moreover, in vitro studies revealed that the overexpression of Cx43 decreases cell growth and induces apoptosis in pituitary tumor cell lines. Our results indicate that the downregulation of Cx43 protein might be involved in the tumorigenesis of most pituitary adenomas and have a potential therapeutic value for pituitary tumor therapy.
Databáze:	MEDLINE
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