Polymorphisms in Human IL4 , IL10 , and TNF Genes Are Associated with an Increased Risk of Developing NSAID-Exacerbated Respiratory Disease.

Autor:	Reigada-Rivera ML; Department of Clinical Biochemistry, University Hospital of Salamanca, 37007 Salamanca, Spain.; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain., Lozano CS; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Microbiology and Genetics, University of Salamanca, 37007 Salamanca, Spain., Rodilla EM; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Allergy, University Hospital of Salamanca, 37007 Salamanca, Spain.; Department of Biomedical Sciences and Diagnostics, University of Salamanca, 37007 Salamanca, Spain., García-Sánchez A; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Biomedical Sciences and Diagnostics, University of Salamanca, 37007 Salamanca, Spain., García-Solaesa V; Department of Clinical Biochemistry, University Hospital of Salamanca, 37007 Salamanca, Spain.; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain., Toledano FL; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Biomedical Sciences and Diagnostics, University of Salamanca, 37007 Salamanca, Spain.; Department of Pediatrics, University Hospital of Salamanca, 37007 Salamanca, Spain., González ID; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Allergy, University Hospital of Salamanca, 37007 Salamanca, Spain.; Department of Biomedical Sciences and Diagnostics, University of Salamanca, 37007 Salamanca, Spain., Isidoro-García M; Department of Clinical Biochemistry, University Hospital of Salamanca, 37007 Salamanca, Spain.; Biomedical Research Institute of Salamanca IBSAL, 37007 Salamanca, Spain.; Department of Medicine, University of Salamanca, 37007 Salamanca, Spain.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2022 Mar 28; Vol. 13 (4). Date of Electronic Publication: 2022 Mar 28.
DOI:	10.3390/genes13040605
Abstrakt:	Background: The role of genetics in non-steroidal anti-inflammatory drugs (NSAID) exacerbated respiratory disease (NERD) is unclear, with different candidates involved, such as HLA genes, genes related to leukotriene synthesis, and cytokine genes. This study aimed to determine possible associations between 22 polymorphisms in 13 cytokine genes. Methods: We included 195 patients (85 with NERD and 110 with respiratory disease who tolerate NSAIDs) and 156 controls (non-atopic individuals without a history of asthma, nasal polyposis (NP), or NSAID hypersensitivity). Genotyping was performed by sequence-specific primer polymerase chain reaction (PCR-SSP). Amplicons were analyzed by horizontal gel electrophoresis in 2% agarose. Results: Significant differences in allele and genotype frequency distributions were found in TNF (rs1800629), IL4 (rs2243248 and rs2243250), and IL10 (rs1800896, rs1800871, and rs1800872) genes in patients with NSAID hypersensitivity. In all cases, the minor allele and the heterozygous genotype were more prevalent in NERD. An association of TNF rs1800629 SNP with respiratory disease in NSAID-tolerant patients was also found. Conclusions: Retrospectively recorded, we found strong associations of NERD with polymorphisms in IL4 , IL10 , and TNF genes, suggesting that these genes could be involved in the inflammatory mechanisms underlying NERD.
Databáze:	MEDLINE
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