Treatment with Ad5-Porcine Interferon-α Attenuates Ebolavirus Disease in Pigs.

Autor:	Senthilkumaran C; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.; Public Health Ontario, Santé Publique Ontario, Toronto, ON M5G 1M1, Canada., Kroeker AL; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.; VIDO-InterVac, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada., Smith G; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada., Embury-Hyatt C; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada., Collignon B; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada., Ramirez-Medina E; Plum Island Animal Disease Center, North-East Area, Agricultural Research Service, U.S. Department of Agriculture, Orient, NY 11957, USA., Azzinaro PA; Plum Island Animal Disease Center, North-East Area, Agricultural Research Service, U.S. Department of Agriculture, Orient, NY 11957, USA., Pickering BS; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB R3E OW2, Canada., Diaz-San Segundo F; Plum Island Animal Disease Center, North-East Area, Agricultural Research Service, U.S. Department of Agriculture, Orient, NY 11957, USA., Weingartl HM; National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB R3E OW2, Canada., de Los Santos T; Plum Island Animal Disease Center, North-East Area, Agricultural Research Service, U.S. Department of Agriculture, Orient, NY 11957, USA.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2022 Apr 08; Vol. 11 (4). Date of Electronic Publication: 2022 Apr 08.
DOI:	10.3390/pathogens11040449
Abstrakt:	Under experimental conditions, pigs infected with Ebola Virus (EBOV) develop disease and can readily transmit the virus to non-human primates or pigs. In the event of accidental or intentional EBOV infection of domestic pigs, complex and time-consuming safe depopulation and carcass disposal are expected. Delaying or preventing transmission through a reduction in viral shedding is an absolute necessity to limit the spread of the virus. In this study, we tested whether porcine interferon-α or λ3 (porIFNα or porIFNλ3) delivered by a replication-defective human type 5 adenovirus vector (Ad5-porIFNα or Ad5-porIFNλ3) could limit EBOV replication and shedding in domestic pigs. Our results show that pigs pre-treated with Ad5-porIFNα did not develop measurable clinical signs, did not shed virus RNA, and displayed strongly reduced viral RNA load in tissues. A microarray analysis of peripheral blood mononuclear cells indicated that Ad5-porIFNα treatment led to clear upregulation in immune and inflammatory responses probably involved in protection against disease. Our results indicate that administration of Ad5-porIFNα can potentially be used to limit the spread of EBOV in pigs.
Databáze:	MEDLINE
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