Dynamic surface tension probe for measuring the concentration of extracellular vesicles.

Autor: Chernyshev VS; Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026, Russian Federation; School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Institutsky per. 9/7, Dolgoprudny, Moscow Region, 141700, Russian Federation. Electronic address: v.chernyshev@skoltech.ru., Chuprov-Netochin RN; School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Institutsky per. 9/7, Dolgoprudny, Moscow Region, 141700, Russian Federation., Tsydenzhapova E; School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Institutsky per. 9/7, Dolgoprudny, Moscow Region, 141700, Russian Federation., Van Devener B; Utah Nanofab, Nano-Scale Imaging and Surface Analysis Lab, University of Utah, 36 S. Wasatch Dr, Salt Lake City, UT, 84112, USA., Leonov S; School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Institutsky per. 9/7, Dolgoprudny, Moscow Region, 141700, Russian Federation., Gorin D; Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Building 3, Moscow, 143026, Russian Federation., Skliar M; The Nano Institute of Utah, University of Utah, 36 S. Wasatch Dr, Salt Lake City, UT, 84112, USA; Department of Chemical Engineering, University of Utah, 50 S. Central Campus Dr, Salt Lake City, UT, 84112, USA.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Jun 18; Vol. 609, pp. 189-194. Date of Electronic Publication: 2022 Apr 10.
DOI: 10.1016/j.bbrc.2022.04.017
Abstrakt: The concentration of extracellular vesicles (EVs) is an essential attribute of biofluids and EV preparations. EV concentration in body fluids was correlated with health status. The abundance of EV secreted by cultured cells into growth medium is vital in signaling studies, tissue and disease models, and biomanufacturing of acellular therapeutic secretome. A limited number of physical principles sensitive to EV concertation have been discovered so far. Particle-by-particle counting methods enumerate individual particles scattering light, modulating the Coulter current, or appearing in EM images. The available ensemble techniques in current use rely on the concentration-dependent signal intensity, as in the case of ELISA. In this study, we propose for the first-time the ensemble-based characterization of EV concentration by dynamic surface tension (DST) probe and demonstrate its implementation. We show that DST measurements agree with the widely used NTA measurements of EV concertation. The proposed method is low-cost and requires only basic laboratory equipment for implementation.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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