| Autor: |
Duong BT; Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, Korea., Than DD; Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, Korea., Ankhanbaatar U; State Central Veterinary Laboratory, Zaisan, Ulaanbaatar, Mongolia., Gombo-Ochir D; State Central Veterinary Laboratory, Zaisan, Ulaanbaatar, Mongolia., Shura G; State Central Veterinary Laboratory, Zaisan, Ulaanbaatar, Mongolia., Tsolmon A; State Central Veterinary Laboratory, Zaisan, Ulaanbaatar, Mongolia., Pun Mok CK; HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China., Basan G; State Central Veterinary Laboratory, Zaisan, Ulaanbaatar, Mongolia., Yeo SJ; Department of Tropical Medicine and Parasitology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea., Park H; Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, Korea. |
| Abstrakt: |
Several novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia in 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two novel genotypes of A(H5N6) subtype in Mongolia during 2018-2019 (A/wildDuck/MN/H5N6/2018-19). Their evolution pattern and molecular characteristics were evaluated using gene sequencing and their pathogenicity was determined using a mouse model. We also compared their antigenicity with previous H5 Clade 2.3.4.4 human isolates by cross-hemagglutination inhibition (HI). Our data suggests that A/wildDuck/MN/H5N6/2018-19 belongs to clade 2.3.4.4h, and maintains several residues associated with mammal adaptation. In addition, our evaluations revealed that their isolates are less virulent in mice than the previously identified H5 human isolates. However, their antigenicity is distinct from other HPAIVs H5 clade 2.3.4.4, thus supporting their continued evaluation as potential infection risks and the preparation of novel candidate vaccines for their neutralization. |
