Co-Operative Biofilm Interactions between Aspergillus fumigatus and Pseudomonas aeruginosa through Secreted Galactosaminogalactan Exopolysaccharide.

Autor:	Ostapska H; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H3A 1Y2, Canada., Le Mauff F; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H3A 1Y2, Canada., Gravelat FN; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H3A 1Y2, Canada., Snarr BD; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H3A 1Y2, Canada., Bamford NC; Program in Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK., Van Loon JC; Program in Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada., McKay G; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC H4A 3J1, Canada., Nguyen D; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada., Howell PL; Program in Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada., Sheppard DC; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.; Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H3A 1Y2, Canada.; Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada.
Jazyk:	angličtina
Zdroj:	Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2022 Mar 24; Vol. 8 (4). Date of Electronic Publication: 2022 Mar 24.
DOI:	10.3390/jof8040336
Abstrakt:	The mold Aspergillus fumigatus and bacterium Pseudomonas aeruginosa form biofilms in the airways of individuals with cystic fibrosis. Biofilm formation by A. fumigatus depends on the self-produced cationic exopolysaccharide galactosaminogalactan (GAG), while P. aeruginosa biofilms can contain the cationic exopolysaccharide Pel. GAG and Pel are rendered cationic by deacetylation mediated by either the secreted deacetylase Agd3 ( A. fumigatus ) or the periplasmic deacetylase PelA ( P. aeruginosa ). Given the similarities between these polymers, the potential for biofilm interactions between these organisms were investigated. P. aeruginosa were observed to adhere to A. fumigatus hyphae in a GAG-dependent manner and to GAG-coated coverslips of A. fumigatus biofilms. In biofilm adherence assays, incubation of P. aeruginosa with A. fumigatus culture supernatants containing de- N -acetylated GAG augmented the formation of adherent P. aeruginosa biofilms, increasing protection against killing by the antibiotic colistin. Fluorescence microscopy demonstrated incorporation of GAG within P. aeruginosa biofilms, suggesting that GAG can serve as an alternate biofilm exopolysaccharide for this bacterium. In contrast, Pel-containing bacterial culture supernatants only augmented the formation of adherent A. fumigatus biofilms when antifungal inhibitory molecules were removed. This study demonstrates biofilm interaction via exopolysaccharides as a potential mechanism of co-operation between these organisms in chronic lung disease.
Databáze:	MEDLINE
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